房颤大鼠心肌血管紧张素II受体表达及青蒿提取物的干预作用  

Myocardial angiotensin II receptor expression in rats of atrial fibrillation and Artemisia annua extract intervention on the expression

作　　者：焦华琛[1] 刘春英[1] 郭丽[1] 彭波[1] 

机构地区：[1]山东中医药大学附属医院心内科,济南250011

出　　处：《国际中医中药杂志》2013年第5期410-412,共3页International Journal of Traditional Chinese Medicine

基　　金：山东省优秀中青年科学家奖励基金资助（项目编号：NO.2010BSA01005）

摘　　要：目的探讨房颤状态下大鼠心肌血管紧张素I型（AT1R）、II型受体（AT2R）mRNA的表达水平以及青蒿提取物对其表达的影响。方法建立房颤大鼠模型，用青蒿提取物进行干预，以卡托普利作为对照组。通过PCR、Western-blot技术观察ATlRmRNA、AT2RmRNA表达以及蛋白表达。结果与对照组（0．36±0．05）相比，模型组大鼠（0．84±0．04）心肌ATlRmRNA的表达增加（P〈0．05）。青蒿组（0．56±0．03）与卡托普利组（0．53±0．04）均可减少房颤大鼠心肌ATlRmRNA的表达（P〈0．05）。卡托普利组对AT，RmRNA的减少趋势较明显，但与青蒿组比较，差异无统计学意义（P〉0．05）。青蒿提取物对于AT2RmRNA的表达未见影响。结论房颤大鼠ATlR的表达明显增加。青蒿提取物可有效减少房颤大鼠心肌ATlR表达。Objective Through establishing a rat model of atrial fibrillation, to study myocardial angiotensin type I （AT1R） and type II receptor （AT2R） mRNA expression levels in of the state atrial fibrillation and Artemisia annua extract on its expression. Methods Rat model of atrial fibrillation was established, Artemisia annua extract was used for intervention and captopril was adopted as controls. AT1R, AT2R mRNA and protein expression were observed by PCR and Western-blot technology. Results Compared with the control group （0.36±0.05） , myocardial ATIR mRNA expression was significantly increased in the modelgroup （0.84±0.04） （P〈0.05） . BothArtemisia annua （0.56±0.03） andcaptopril （0.53±0.04） could significantly reduce the myocardial ATIR mRNA expression in the atrial fibrillation rats （P〈0.05） . Captopril showed obvious ATIR mRNA reduction trend, but there was statistical significance compared with Artemisinic extract （P〉0.05）. Artemisinic extract showed no impact on AT2R mRNA expression. Conclusion ATlR was closely related to the incidence of atrial fibrillation. AT1R expression was significantly increased in atrial fibrillation rat. The artemisinic extract can be effectively reduced fibrillation myocardial ATIR expression, which may link with its artemisinic antiarrhythmic mechanism.

关 键 词：房颤 血管紧张素II受体 AT1R 青蒿提取物 

分 类 号：R259[医药卫生—中西医结合]