赛拉嗪麻醉对大鼠大脑皮质神经细胞游离钙离子浓度的影响  

Effects of Xylazine on free calcium ion concentration in rat cerebral cortex nerve cells

作　　者：李志强[1] 李小波[1] 石星星[1] 尹柏双[1] 范宏刚[1] 王洪斌[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2013年第4期3-4,8,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30671552;30972227);哈尔滨市青年科技创新人才基金项目(2010RFQNX105)

摘　　要：为了研究赛拉嗪麻醉对大鼠大脑皮质神经细胞游离钙离子浓度的影响,将15只SD大鼠在无菌条件下处死,分离脑组织、制备脑神经细胞悬液,分为对照组、KCl组、赛拉嗪低剂量组和赛拉嗪高剂量组。用Fluo-3/AM负载,除对照组外其他组使用50mmol/L的KCl刺激,药物组加入药物,然后用流式细胞仪检测钙离子荧光强度。结果显示,对照组荧光强度最低,赛拉嗪低剂量组较KCl组显著增加(P<0.05),赛拉嗪高剂量组较KCl组极显著增加(P<0.01)。结果表明,Ca2+是赛拉嗪全麻作用的主要靶位之一,而且其中枢抑制效应与增加神经细胞内[Ca2+]i有关。In order to investigate the effects of Xylazine on free calcium ion concentrationin rat cerebral cortex nerve ceils , 15 SD rats were sacrificed under sterile conditions, iso-lated brain tissue, preparated brain cell suspension ,then divided them into the control gro-up, the KC1 group, the low-dose of Xylazine group and the high-dose of Xylazine group. Loaded with Fluo-3/AM, 50 mmol/L KC1 stimulate other groups in addition to the control group, drug group with drugs, and measured calcium fluorescence intensity by flow cyto-metry. The results showed that fluorescence intensity of the control group was the lowest, compared with the KC1 group, the low dose group of Xylazine increased significantly （P〈0.05） ,and the high dose of Xylazine group was extraordinarily higher （P〈0.01）. Fromthe results we can conclude that, Ca^2＋ is one of the main anesthetic targets of the Xylazi-ne, and the central inhibitory effect associated with the increase of [Ca^2＋ ]i in nerve cells.

关 键 词：赛拉嗪 神经细胞 CA2+ Fluo-3/AM 流式细胞仪 

分 类 号：S859.791[农业科学—临床兽医学]