君子兰试管苗叶片植株再生的影响因素研究  

Study on Factors Influencing Plant Regeneration of Clivia miniata Leaf

作　　者：关丽霞[1] 

机构地区：[1]辽宁农业职业技术学院生物技术系,辽宁营口115009

出　　处：《北方园艺》2013年第9期114-116,共3页Northern Horticulture

摘　　要：以君子兰试管苗叶片为试材,研究了不同培养基、叶片不同部位、增殖代数等因素对其植株再生的影响。结果表明:君子兰试管苗叶片基部再生能力最强,其最佳诱导分化培养基为MS+ZT 1.5mg/L+KT 0.5mg/L+NAA 0.5mg/L;组织培养增殖1～7代试管苗叶片的再生能力较强,随之培养再生能力减弱,10代后再生能力最弱;培养基1/2MS+IBA 1.0mg/L为试管苗生根最适宜的培养基,生根率达98%以上。Taking the leaf of Clivia miniata as material, the effects of different culture mediums, different leaf parts and muhiplieation algebra on the plant regeneration in vitro was studied. The results showed that the in vitro Clivia miniata leaf base regeneration capacity was the strongest,and the best differentiation medium was MS＋ZT 1.5 mg/L＋KT 0. 5 mg/L＋NAA 0. 5 mg/L;tissue culture proliferation 1-7 generation of the in vitro Clivia miniata leaf regeneration ability was stronger, then culture regeneration ability was abate, 10 generations after the regenerative capacity of the weakest;the best rooting medium for the in vitro plant was 1/2MS＋IBA 1.0 mg/L,with rooting rate more than 98%.

关 键 词：君子兰 叶片 植株再生 

分 类 号：S682.13[农业科学—观赏园艺]