人脐血源基质细胞与骨髓基质细胞对造血功能损伤修复作用的比较研究  

Comparison of the effect of the recovery of hematopoiesis of human umbilical cord blood-derived stromal cells with that of human bone marrow stromal cells

作　　者：樊智敏[1] 张曦[1] 刘耀[1] 彭贤贵[1] 高力[1] 

机构地区：[1]第三军医大学新桥医院血液科全军血液病中心重庆市医学重点学科,重庆400037

出　　处：《中国输血杂志》2013年第3期106-109,共4页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金课题(81070388);重庆市自然基金重点课题(2009BA5056)

摘　　要：目的比较人脐血源基质细胞(hUCBDSCs)与骨髓基质细胞(hBMSCs)对造血功能损伤的修复作用。方法取63只BALB/c小鼠均给予6.0 Gy60Co射线全身照射,随机分为hUCBDSCs组(n=21)、hBMSCs组(n=21)和对照组(生理盐水组)(n=21),分别于移植后1、3、5、7、10、14、21 d检测1次外周血细胞,于照射后7和21 d分别制备股骨骨髓涂片观察骨髓细胞形态学改变,收集小鼠骨髓细胞并作集落培养。结果 3组小鼠移植后白细胞、血小板、红细胞在3～5 d降至最低,hUCBDSCs组白细胞和血小板均从7 d开始回升,10 d后回升速度与程度提高,21 d时回升接近未照射水平,hBMSCs组从7 d开始回升,回升趋势与hUCBDSCs组相似,21 d后回升程度低于未照射水平,对照组从7 d后开始回升,回升较慢,21 d回升程度低于实验组(P<0.05);hUCBDSCs组,hBMSCs组及对照组红细胞均在3 d时降至最低,21 d后恢复,但实验组较对照组回升更快,(P<0.05),hUCBDSCs组与hBM-SCs无甚差异(P>0.05)。集落培养CFU-E、BFU-E、CFU-GM、CFU-GMEM,hUCBDSCs组21 d为(113±6)、(167±6)、(131±6)、(14±4)个,hBMSCs组(46±6)、(141±11)、(118±18)、(10±1)个,对照组为(87±3)、(122±4)、(84±7)、(5±1)个(P<0.05)。hUCBDSCs组所有小鼠均存活。结论 hUCBDSCs较hBMSCs修复造血功能损伤和血小板恢复的作用更佳,hUCBDSCs移植是1种安全有效的促进造血损伤修复的方法。Objective To compare the effect of the recovery of hematopoiesis of human umbilical cord blood-derived stromal cells with that of human bone marrow stromal cells(hBMSCs).Methods A total of 63 BALB/C mice were given 6.0Gy 60Co rays total body irradiation and randomly assigned into irradiation control group(n=21),hBMSCs group(n=21),hUCBDSCs group(n=21).After the transplantation,the changes in peripheral blood,colony count,bone marrow biopsy and pathology were observed on 1 d,3 d,5 d,7 d,10 d,14 d and 21 d,respectively.Results White blood cell,platelet and red blood cell declined on 3~5days after all the transplantation,picked up on the 7th day,the hUCBDSCs group was higher than that in hBMSCs group and control group(P<0.05),and was fastest recovery in the 3 groups(P<0.05).The CFU-E,BFU-E,CFU-GM,CFU-GMEE counts in hUCBDSCs group on 21days were(113±6),(167±6),(131±6),(14±4),which were also higher than that in hBMSCs group and control group(P<0.05),and all the mice were survival.The CFU-E,BFU-E,CFU-GM,CFU-GMEE counts in hBMSCs group on 21days were(46±6),(141±11),(118±18),(10±1) and were(87±3),(122±4),(84±7),(5±1)in control group.The results among the 3 groups were statistically significant different.(P<0.05) Conclusion hUCBDSCs is better than hBMSCs on the effect of hemopoiesis repair and thombocytopoiesis.hUCBDSCs transplantation should be looked as a safe and effective way for recovery of hematopoietic microenvironment.

关 键 词：人脐血基质细胞 人骨髓基质细胞 造血修复 造血微环境 外周血细胞 集落培养 BALB C小鼠 

分 类 号：R457.7[医药卫生—治疗学] R331.2[医药卫生—临床医学]