PCR-SSP技术对羊水细胞ABO血型的基因鉴定  

Identification of fetal ABO blood groups by analyzing amniotic fluid cells via PCR-SSP

作　　者：马欣[1] 陈江[2] 

机构地区：[1]宜宾市中心血站,四川宜宾644000 [2]宜宾市第二人民医院

出　　处：《中国输血杂志》2013年第3期142-144,共3页Chinese Journal of Blood Transfusion

基　　金：四川省卫生厅资助项目(20100583);宜宾市科技局项目(2010SF011)

摘　　要：目的通过PCR-SSP基因技术检测胎儿羊水细胞ABO血型基因型,产前诊断胎儿ABO血型。方法选取了6名孕16 W以上的孕妇,抽取羊水细胞并进行分离,提取羊水细胞DNA,运用PCR-SSP技术分析其ABO血型基因型,并通过出生后的脐带血的血型鉴定进行确认。结果 6例羊水标本均通过PCR-SSP方法检测出了ABO血型的基因型;该6名胎儿的脐带血的ABO血型与羊水细胞的血型一致。结论 PCR-SSP技术可以准确地检测胎儿羊水细胞的ABO血型。Objective To identify fetal ABO blood groups by analyzing amniotic fluid cells via PCR-SSP.Methods Six pregnant women with gestational age between 16 weeks and 25 weeks were selected.DNA was taken in amniotic fluid cells from these pregnant women and then ABO genotypes of amniotic fluid cel1s were detected by PCR-SSP.And the ABO genotypes were conformed by detecting the cord blood.Results The ABO genotypes were identified from the 6 samples of amniotic fluid cells,and the results were in accordance with cord blood.Conclusion Fetal ABO blood group can be accurately detected out by amniotic fluid cells.

关 键 词：ABO血型 羊水细胞 PCR-SSP法 

分 类 号：R457.11[医药卫生—治疗学]