HPLC法测定感冒止咳颗粒中葛根素的含量  

HPLC in Determination of Cold Zhike Granules of Puerarin Content

作　　者：刘海霞[1] 杨志伟[1] 邹文斌[1] 

机构地区：[1]松原市药品检验所,吉林松原138000

出　　处：《亚太传统医药》2013年第5期52-54,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:建立以高效液相色谱法测定感冒止咳颗粒中葛根素含量的方法。方法:ZORBAX SB-C18(250mm×4.6mm,5μm),流动相为甲醇-水(28∶72),流速为1.0mL/min,检测波长为250nm,柱温为30℃。结果:葛根素在0.080 1～1.602 4μg/mL范围内呈良好的线性关系。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。Objective：To establish a high performance liquid chromatography determination of puerarin in cold cough particle con tent of method. Methods：ZORBAX SB-C18 （250mm×4.6mm,5μm）, mobile phase was methanol and water （28 ：72）, flow rate is 1.0mL/min, detection wavelength was 250nm, column temperature was 30℃. Results： Puerarin of 0. 080 1 - 1. 602 4μg/mL .showed good linear relationship. Conclusion：This method is simple and accurate, good separating effect, wide linear range, high sensitivity, and can be used for the quality control of the product.

关 键 词：高效液相色谱法 感冒止咳颗粒 葛根素 含量 

分 类 号：R284.1[医药卫生—中药学]