银杏叶提取物联合骨髓间充质干细胞治疗(I型)糖尿病大鼠疗效观察  

Observation on the Effect of Ginkgo Biloba Extract Plus Bone Marrow Derived Stroma Cell on a Rat Model of(type I) Diabetes

作　　者：安立峰[1] 石琳琳[2] 李琳[1] 崔娜[1] 修倩[1] 王艳姝[3] 

机构地区：[1]吉林大学中日联谊医院耳鼻喉科,吉林长春130033 [2]吉林大学中日联谊医院内分泌科,吉林长春130033 [3]吉林大学第一医院麻醉科,吉林长春130031

出　　处：《现代生物医学进展》2013年第6期1030-1032,1058,共4页Progress in Modern Biomedicine

基　　金：教育部新世纪人才计划资金资助(4500912051)

摘　　要：目的:探讨银杏叶提取物联合骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)治疗(I型)糖尿病大鼠的效果。方法:用腹腔内注射链脲酶菌素方法造模I型糖尿病wistar大鼠,BMSCs治疗(1×106/个)(DM)大鼠的同时,给予银杏叶提取物(Ginkgo biloba extract,GBE)治疗20天,同时设立单纯BMSCs治疗组,单纯GBE治疗组,糖尿病模型组以及正常对照组。分时段监测各组大鼠随机血糖、胰岛素、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)以及超氧化物歧化酶(SOD)水平。结果:与其它各组相比,与正常对照组相比DM组血糖水平明显高于GBE组和BMSCs组,BMSCs联合GBE治疗组的大鼠MDA水平明显降低于各治疗组,胰岛素、GSH-Px和SOD水平则明显高于其他对照治疗组。结论:BMSCs联合GBE治疗I型DM大鼠,可以明显降低大鼠的血糖和氧化应激水平,其效果好于单独银杏叶提取物治疗或BMSCs治疗。Objective： To explore the potential treatment effect of type I diabetes by BMSCs with GBE. Methods： In the treatment of diabetic rats, anti-oxidant GBE has been carried on to exploit a better condition so as to measure in the follows： blood glucose, insulin, MDA, GSH-Px, SOD. Results： Compared with other groups, the treatment groups＇ blood glucose was lower than the normal control. The experi-mental group of combine treatment of BMSCs and GBE showed the rise of insulin GSH-Px and SOD, with the decrease of blood glucose and MDA as compared with the other groups. Conclusions： BMSCs combined with GBED in treating DM rats can reduce the level of blood glucose and oxidative stress, better than treatment with anti-oxidant or stem cell alone.

关 键 词：骨髓间充质干细胞 银杏叶提取物 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]