比较口服不同亲和力重组耻垢分支杆菌调节免疫的差异  

Comparison of Immune Differences with Oral Administrated Different Affinity of Recombinant Mycobacterium Smegmatis

作　　者：解婷[1] 薛莹[2] 樊爱琳[3] 简文[1] 

机构地区：[1]第四军医大学附属西京医院呼吸与危重症医学科,陕西西安710032 [2]第四军医大学附属西京医院放疗科,陕西西安710032 [3]第四军医大学附属西京医院检验科,陕西西安710032

出　　处：《现代生物医学进展》2013年第8期1435-1438,1477,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30871114)

摘　　要：目的:Th1与Th2的平衡紊乱是过敏性哮喘的免疫学基础,本研究通过给予小鼠口服免疫不同亲和力屋尘螨抗原Der p2重组耻垢分枝杆菌,来比较其调节免疫应答的差异。方法:1,分别给予6-8周BALB/c小鼠口服接种Mycobacterium Smegmatis(M.S)、pCW-Der p2-rM.S及pCW-Der p2-PEB1-rM.S,在末次免疫后第14、28、56天分别用夹心ELISA法测定小鼠血清及脾脏淋巴细胞培养上清中IFN-γ、IL-2及IL-4的含量。结果:夹心ELISA结果显示,无论是血清还是脾脏淋巴细胞培养上清中,各组IFN-γ、IL-2水平逐渐升高,IL-4水平逐渐降低,8周时更明显,pCW-Der p2-PEB1-rM.S组与M.S、pCW-Der p2-rM.S组相比有明显统计学差异。体外给予Der p2蛋白刺激后,pCW-Der p2-rM.S及pCW-Der p2-PEB1-rM.S组变化更加明显,而M.S组无明显变化。结论:小鼠口服免疫pCW-Der p2-PEB1-rM.S能明显提高Th1型免疫反应,与pCW-Der p2-rM.S组相比有统计学学差异。三种不同亲和力重组耻垢分枝杆菌经口服后均能诱导小鼠产生Th1型免疫应答,且pCW-Der p2-rM.S及pCW-Der p2-PEB1-rM.S诱导产生的Th1优势应答具有Der p2抗原特异性。本研究弥补了粘膜型疫苗低亲和力的不足,为新型口服疫苗的制备和改进创造了条件。Objective： Thl and Th2 balance disorder is the basic immunology of allergic asthma. In this study, we mainly investigate the immune response differences for BALB/c mice with oral administrated different affinity of Mycobacterium Smegmatis. Methods： Mice were killed at 14,28,56 days after intragastric administrated different affinity of Mycobacterium Smegmatis, measured serum and spleen lymphocyte culture supematant by ELISA, respectively. Results： The ELISA showed that with the growth of time, IFN-γ and IL-2 were gradually increased, IL-4 were gradually decreased. All the change reached the peak on the 56th day （P〈 0.05）. The groups of pCW-Der p2-PEBI-rM.S and pCW-Der p2-rM.S showed significant difference compared with M.S. group （P〈 0.05）, after stimulating with Der p2 protein, more obvious change could be observed in the two groups. Conclusion： All groups of Mycobacterium Smegmatis took orally could induce Thl-type immune response, and the response produced by pCW-Der p2-PEBI-rM.S and pCW-Der p2-rM.S was the specific response to Der p2. There showed a significant difference for pCW-Der p2-PEBI-rM.S compared with pCW-Der p2-rM.S group （P〈 0.05）. This study to make up for a insufficiency of mucosal vaccine with low affinity, and create a conditions for new oral vaccine construction and improvement.

关 键 词：Derp2 PEB1 耻垢分枝杆菌 口服疫苗 TH1 TH2 

分 类 号：Q95-3[生物学—动物学] R562.25[医药卫生—呼吸系统]