甘南牦牛NGB基因的克隆及序列分析  

Cloning and sequence analysis of NGB gene of Gannan yak

作　　者：石宁宁[1,2] 杜晓华[2,2] 罗玉柱[3,2] 曹亮[2] 刘霞[1,2] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃农业大学动物医学院,甘肃兰州730070 [3]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《西北农林科技大学学报（自然科学版）》2013年第4期14-20,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：甘肃省自然科学基金项目(1107RJZA148);甘肃省教育厅研究生导师项目(1102-04)

摘　　要：【目的】对甘南牦牛NGB基因进行克隆和序列分析,同时探讨NGB的生理功能。【方法】采用TA克隆法克隆甘南牦牛NGB基因,运用多种软件对其基因序列及编码产物的结构、功能进行了生物信息学分析。【结果】甘南牦牛NGB基因全长3 844bp,包括4个外显子和3个内含子,其内含子中存在2个反向重复序列和1个正向重复序列;牦牛NGBCDs区全长456bp,其编码产物是由151个氨基酸残基组成的可溶性蛋白质,分子质量约为16 876.36u,理论等电点为4.86,推测NGB编码产物可能在牦牛物质运输和结合、能量代谢等过程中发挥着重要作用;系统发育树分析表明,甘南牦牛NGB与牛、藏羚羊等物种之间存在较高的同源性。【结论】甘南牦牛NGB基因的成功克隆,为进一步研究牦牛NGB的遗传特性和生理功能奠定了基础。[Objective] In this study,the NGB gene of Gannan yak was cloned and its sequence was analyzed. The physiological function of the NGB was explored as well. [Method] NGB gene of Gannan yak was cloned by TA cloning. The NGB gene sequence, structure, function and its encoded amino acid se quence were analyzed with biological information. [Result] The full-length of NGB gene was 3 844 bp,including four exons and three introns. There were two inverted repeat sequences and a direct repeat sequence in intron. The coding region of NGB gene was 456 bp,encoding a protein of 151 amino acids with a molecular weight of 16 876.36 u and pI of 4.86. The deduced encoded product was a soluble protein,playing an important role in the process of transport, binding and energy metabolism. Phylogenetic analysis showed that there was a higher homology between Gannan yak and Bos taurus,Pantholops hodgsonii. [Conclusion] This study paved the way for future study of genetic characteristics and physiological mechanism of NGB gene of Gannan yak.

关 键 词：甘南牦牛 脑红蛋白 基因克隆 生物信息学分析 

分 类 号：S823.85[农业科学—畜牧学]