心力衰竭过程中调节性T细胞的变化及意义  

Changes and significance of regulatory T lymphocyte in rats with heart failure

作　　者：姚红[1] 王艳红[1] 刘慧荣[2,3] 

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原市030001 [2]山西医科大学生理学教研室,太原市030001 [3]首都医科大学病理生理学教研室,首都医科大学心脏病学系

出　　处：《中国心血管病研究》2013年第4期293-295,共3页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金项目（项目编号：30900585）;山西医科大学博士启动基金（项目编号：03200803）

摘　　要：目的观察大鼠心力衰竭过程中外周血调节性T（Treg）~胞的变化规律并探讨其意义。方法采用腹主动脉缩窄法制备大鼠心力衰竭模型，定期应用流式细胞术检测实验大鼠外周血CD4’CD25Yreg／CD4VY和CD4＋CD25＋F0xp3叮reg／CD4＋T细胞的比例。结果①大鼠经腹主动脉缩窄术处理8周时，心功能明显下降，心衰模型建立成功。②在腹主动脉缩窄术处理4周、8周时，心衰组大鼠外周血CD4’CD25＋Treg／CD4＋T和CD4＋CD25＋Foxp3叩reg／CD4CF比例较同期sham组明显降低（P〈0．05）；在处理12周、16周时，其比例与sham组差异无统计学意义（P〉0．05）。结论心衰发病过程中Treg细胞明显下调。Treg细胞可能成为心衰免疫调节治疗的新靶点。Objective To investigate the changes and significance of regulatory T（Treg） cells in rats with heart failure （HF）. Methods Heart failure in rats was induced by constricted the abdominal aorta. The percentage of the peripheral blood Treg was detected by flow cytometry. Results In HF group, the percentage of CD4＋CD25＋ Treg and CD4＋CD25＋Foxp3＋Treg in peripheral blood were considerably decreased as compared with sham group in rats at 4 and 8 weeks after treatment （P〈0.05）. Then, the percentage of the peripheral blood CD4＋ CD25＋Treg and CDg＋CD25＋Foxp3＋Treg were no significant differences between HF and sham groups （P〉0.05）. Conclusion In rats with HF, the peripheral CDg ＋CD25 ＋Treg are reduced, and their functional properties compromised. Treg cells may become a new target for immunotberapy in heart failure.

关 键 词：心力衰竭 调节性T细胞 免疫调节 

分 类 号：Q95-33[生物学—动物学]