RP-HPLC法测定短筒兔耳草中松果菊苷  

Determination of echinacoside in Lagotis brevituba by RP-HPLC

作　　者：宋杰[1,2] 崔保松[1] 李帅[1] 马林[1] 张瑛[1] 石建功[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050 [2]北京协和制药二厂,北京102600

出　　处：《药物评价研究》2013年第2期132-134,共3页Drug Evaluation Research

基　　金：<中国药典>2010版一部标准研究项目(YS-204)

摘　　要：目的建立短筒兔耳草中松果菊苷的RP-HPLC测定方法。方法短筒兔耳草经流动相超声提取后,用RP-HPLC法测定。采用Alltech Prevail C18(250mm×4.6mm,5μm)色谱柱,流动相为乙睛-甲醇-1%醋酸(10:15:75),体积流量1.0mL/min,检测波长334 nm,柱温为室温。结果松果菊苷进样量在0.46～18.40μg内与峰面积呈良好的线性关系(r=0.999 9);平均回收率为101.11%,RSD为2.09%(n=6)。结论采用RP-HPLC测定短筒兔耳草药材中松果菊苷的含量,该方法准确、快速、简便,重复性好,适用于短筒兔耳草中松果菊苷的质量控制。Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C1s column （250 mm× 4.6 mm, 5 μm） with acetonitrile-methanol-l% acetic acid （10：15：75） as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46-18.40μg （r=0.999 9）. The average recovery of echinacoside was 101.11% and RSD was 2.09% （n=6）. Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control ofL. brevituba.

关 键 词：短筒兔耳草 松果菊苷 RP-HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]