甘草水煎剂对变异链球菌黏附影响的体外实验  

Effects of licorice decoction on the adherence of Streptococcus mutans in vitro

作　　者：黄萍[1] 万呼春[1] 田婷玉[1] 何永红[1] 

机构地区：[1]口腔疾病研究国家重点实验室华西口腔医院特诊科,四川大学成都610041

出　　处：《国际口腔医学杂志》2013年第3期285-287,共3页International Journal of Stomatology

摘　　要：目的初步探讨甘草水煎剂对体外培养的变异链球菌黏附能力的影响及其机制。方法将甘草用水煎煮制备成水煎剂,按所含甘草的不同质量浓度25、50、100 g.L-1分为A、B、C三组,不含甘草水煎剂的设为对照组。实验在玻璃试管中进行,分别收集每管中紧密黏附、非黏附和松散黏附的细菌,计算每组细菌的黏附率;用蒽酮法测定各组细菌合成水不溶性胞外多糖的含量。结果 A组、B组、C组和对照组的细菌黏附率分别为70.2%、40.1%14.2%、87.1%,各组细菌合成的水不溶性胞外多糖的质量浓度分别为0.28、0.25、0.13、0.30 g.L-1。结论甘草水煎剂能够明显抑制变异链球菌对光滑表面的黏附,该抑制作用存在剂量-效应关系,可能与其抑制细菌水不溶性胞外多糖的合成有关。Objective To determine the effects of licorice decoction on the adherence of Streptococcus mutans in vitro and the possible mechanisms. Methods Licorice decoction was from self-preparation. According to the licorice decoction＇s mass concentration 25, 50 and 100 g·L^（-1）, the experiment were divided into A, B and C groups. The group not containing decoction was set as negative control group. The experiment was performed in the glass tubes. Bacteria adhering tightly, loosely or not adhering to the glass were collected separately to determine bacteria＇s adhesion rates. The quantity of water insoluble extraeellular polysaccharides produced by bacteria was measured by anthrone method. Results Bacteria＇s adhesion rates of A, B, C and negative control groups were 70.2%, 40.1%, 14.2% and 87.1% respectively. Water insoluble extraeellular polysaccharides＇s mass concentration of each group was 0.28, 0.25, 0.13, 0.30 g·L^（-1）. Conclusion Licorice decoction has inhibitory potential against the adherence of Streptococcus mutans to smooth surface. The inhibition was dose-dependent, and may be relevant with the inhibition of synthesis of water insoluble extracellular polysaccharides.

关 键 词：甘草 变异链球菌 黏附 

分 类 号：R285.5[医药卫生—中药学]