奶牛乳房炎病原菌的分离和PCR方法快速鉴定  

Pathogens Isolation From Bovine Mastitis Samples and the Rapid Identification by PCR Method

作　　者：甘露[1] 李姝[1] 徐君[1] 黄品[1] 蔡亚非[1] 王根林[2] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全安徽省高校省级重点实验室,安徽芜湖241000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《安徽师范大学学报（自然科学版）》2012年第4期344-350,共7页Journal of Anhui Normal University(Natural Science)

基　　金："十二五"国家科技计划课题(2011BAD28B02;2012BAD12B10);安徽省自然科学基金(1208085MC41);安徽省科技人员服务企业计划项目(10020303043);江苏省农业三项工程课题(SX2011-189);2011年度芜湖市科技计划项目

摘　　要：奶牛乳房炎是奶牛养殖场中最严重的疾病之一.为了确定奶牛乳房炎主要致病菌,本实验对采自养殖场临床乳房炎40个病例的乳样,用传统细菌培养方法分离奶牛乳房炎病原菌进行形态学鉴定;用纯化培养后细菌单菌落提取DNA作为模板,利用已报道的细菌通用引物,采用PCR方法进行分子鉴定.结果共分离得到10种主要致病菌,它们是嗜酸氧化硫硫杆菌,琼氏不动杆菌,农杆菌,蜡状芽孢杆菌,地衣芽胞杆菌,芽孢杆菌,大肠杆菌,克雷伯菌,假单胞菌,金黄色葡萄球菌.本研究成功建立了一种快速、特异性强的奶牛乳房炎致病菌的鉴定方法.Cow mastitis is one of the most important serious problems in the dairy farms. It is caused by many different microorganisms. In this study, forty milk samples were collected from clinical mastitis cows. Mastitis pathogens are isolated by the traditional bacterial culture method. DNA was extracted from bacterial colonies. Then the types of bacteria was identified by using Polymerase Chain Reaction method （PCR）. The main pathogens were isolated, including Acidithiobacillus thiooxidans, Acinetobacter junii, Agrobacterium tumefaciens, Bacillus cereus, Bacillus licheniformis, Bacillus sp., Enterobacter sp., Klebsiella sp., Pseudomonas sp., Staphylococcus aureus. Successfully establish a rapid, sensitive and specific identification method of mastitis pathogens. It also lays the technical foundation for the rapid diagnosis of dairy mastitis cows.

关 键 词：奶牛乳房炎 致病菌 细菌培养 PCR 

分 类 号：Q7[生物学—分子生物学]