酸萃取-氢化物发生-原子荧光光谱法快速测定血镉  

Quick determination of Cadmium content in blood by acid extraction-HG–AFS

作　　者：李坤[1] 胥艳[1] 郭文静[1] 

机构地区：[1]河南省开封市疾病预防控制中心,河南开封475000

出　　处：《中国卫生检验杂志》2013年第4期821-823,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立酸萃取-氢化物发生-原子荧光光谱法测定血中镉的方法。方法:微量血样经酸萃取、离心后,取上清液直接测定,反应产生的镉的氢化物用AFS-920双道原子荧光光谱仪进行定量测定。结果:本方法前处理简单易行,检测结果准确度、灵敏度高、精密度好,在0.00μg/L～0.50μg/L浓度范围内,呈良好的线性关系,检出限为3.75 ng/L,相关系数r=0.9997,样品的加标回收率在85.5%～102.3%,RSD为7.09%。结论:该方法是一种简便、快速、准确、可靠的方法,适用于血样中镉的测定。Objective：To develop a quick method for the determination of Cadmium in blood by acid extraction-HG-AFS.Methods： The micro-blood samples were prepared by acid extraction and centrifugation,then the supernatant was determined directly with HG-AFS.Results： A good Linear relationship between the cadmium content and fluorescence intensity was found in the range of 0.00 μg/L^0.50 μg/L with the detection limit of 3.75 ng/L.The related coefficient,recoveries and RSD were 0.9997,85.5%~102.3%,7.09%,respectively.Conclusion： The results indicated that the method was convenient,rapid,accurate,reliable,sensitive and suitable for measurement of cadmium in blood.

关 键 词：酸萃取 氢化物发生 原子荧光光谱法 血镉 

分 类 号：O657.31[理学—分析化学]