Micro-RNA-122在不同时期慢性乙肝患者外周血浆中表达变化的研究  

Liver-specific microRNA-122 levels in different phases of patients with chronic hepatitis B virus infection

作　　者：李祥云[1] 陈兆军[1] 潘峰[1] 刘玉华[1] 黄玲玲[1] 楼洪萍[1] 

机构地区：[1]杭州师范大学附属医院检验科,杭州310015

出　　处：《中国卫生检验杂志》2013年第4期874-876,879,共4页Chinese Journal of Health Laboratory Technology

基　　金：杭州市医药卫生科技计划项目(20100633B17)

摘　　要：目的:观察在慢性乙型肝炎四个不同发病时期中,人外周血浆中肝脏特异性miRNA-122表达量的变化,探讨miRNA-122成为慢性乙型肝炎潜在的辅助诊断检测标志物的可能性和意义。方法:收集80例患者,采用实时荧光定量PCR法和化学发光法等方法对miRNA-122、HBV-DNA、HbeAg、HbsAg和ALT进行测定,对结果进行比较和线性相关分析。结果:miRNA-122在四个时期表达量均明显升高,尤其在免疫耐受期和免疫清除期升高显著(P<0.01);miRNA-122与HbeAg和HBV-DNA相关性不强(r=0.263,r=0.171),与HbsAg和ALT相关性较强(r=0.597,r=0.767)。结论:miRNA-122和乙肝病毒复制可能没有关系,但对于乙肝的辅助诊断、评估及健康管理等具有重要的意义。Objective：To observe miRNA-122 levels in plasma during four different phases of patients with chronic hepatitis B virus infection and explore the possibility and meaning of miRNA-122 serving as potential biomarkers for the diagnosis and prognosis of corresponding clinical conditions.Methods： MiRNA-122,HBV-DNA,HbeAg,HbsAg and ALT levels were mainly detected with real time fluorescence quantitative PCR（FQ-PCR） and chemiluminescence methods in 80 HBV-infected patients.The results were compared and analyzed with linear correlation analysis.Results： MiRNA-122 levels were obviously high during four different phases,especially in immune tolerant phase and in immune clearance phase,showing a significant difference（P0.01）.Meanwhile,there was not a significant linear correlation between miRNA-122 levels and HbeAg and HBV-DNA levels（r=0.263,r=0.171）.But there was a significant linear correlation between miRNA-122 levels and HbsAg and ALT levels（r=0.597,r=0.767）.Conclusion： Maybe there is no relation between miRNA-122 and the viral replication.But miRNA-122 was still important to improve the diagnosis,assessment and health management of patients with HBV infection.

关 键 词：乙肝病毒(HBV) miRNA-122 免疫耐受期 免疫清除期 非活动期 再活动期 

分 类 号：R512.62[医药卫生—内科学]