系统性红斑狼疮患者血清肽酰基精氨酸脱亚胺酶4水平及意义  

Level and significance of serum peptidylarginine deiminase type 4 in patients with systemic lupus erythematous

作　　者：王增玲[1] 邢广群[1] 李春梅[1] 

机构地区：[1]青岛大学医学院附属医院黄岛院区肾内科,山东青岛266555

出　　处：《临床荟萃》2013年第5期515-518,共4页Clinical Focus

摘　　要：目的通过检测肽酰基精氨酸脱亚胺酶4(PAD4)在系统性红斑狼疮(SLE)患者血清中的水平,探讨其在SLE发病机制中的作用。方法收集SLE患者22例,其中活动期患者14例,缓解期患者8例;类风湿性关节炎(RA)患者活动期、缓解期各11例(阳性对照组),慢性肾脏病(CKD)患者8例(疾病对照组),正常对照人群(正常对照组)12例。用ELISA的方法检测血清中PAD4的水平。并研究其与SLE患者中的SLE-DAI、24小时尿蛋白定量、血沉的关系。结果血清PAD4水平在SLE患者活动期及缓解期皆明显高于正常对照组,75.99(60.44,99.16)kU/L,76.29(62.79,119.14)kU/L vs 32.58(26.13,41.46)kU/L(P均<0.01),活动期与缓解期之间差异无统计学意义(P>0.05)。SLE组患者与CKD组患者血清肌酐水平差异无统计学意义(P>0.05),但SLE患者血清PAD4水平无论活动期还是缓解期皆显著高于CKD患者75.99(60.44,99.16)kU/L,76.29(62.79,119.14)kU/L vs 53.11(47.69,58.83)kU/L(均P<0.05)。结论 PAD4在SLE患者中呈现持续高水平的表达,可能参与了SLE的发生、发展。Objective To detect the serum level of peptidylarginine deiminase type 4（PAD4） in patients with systemic lupus erythematous（SLE） ,and explore its pathogenic effect in the disease. Methods 14 patients with SLE on active stage and 8 patients with SLE on remission stage were enrolled in this study. 11 patients with rheumatic arthritis （RA） on active stage and 11 patients with RA on remission stage were considered as positive controls. 8 patients with primary chronic kidney disease（CKD） were considered as disease controls. 12 healthy volunteers were considered as normal controls. All of their serum samples were collected. Serum PAD4 was detected using commercial ELISA kits. The association between serum PAD4 and SLE-DAI,24-hour urinary protein excretion,erythrocyte sedimentation rate （ESR） in SLE patients was further investigated. Results The serum levels of PAD4 on active stage and remission stage of SIzE patients were respectively elevated than that of the normal controls, 75.99 （60.44,99.16） kU/L, 76.29 （62.79,119.14） kU/L vs 32.58（26.13,41.46） kU/L（bothPd0.01）;there were no significant difference between active and remission stage of SLE patients （ P〈 0.05）. Serum creatinine between SLE and CKD patients showed no significant difference（ P 〈0.05）. The serum level of PAD4 in patients of SLE on active and remission stage were higher than that in CKD group,75.99（60.44,99.16） kU/L,76. 29（62. 79,119.14） kU/L vs 53.11（47.69,58.83） kU/ L（both P 〈0.05）. Conclusion PAD4 was kept a higher level in SLE patients,it might involve in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 精氨酸酶 瓜氨酸 

分 类 号：R593.241[医药卫生—内科学]