超高效液相色谱法快速检测地表水中丁基黄原酸  

Rapid Analysis of Butylxanthic Acid in Surface Water by Ultra Performance Liquid Chromatography

作　　者：彭涛 王超[2] 吕怡兵[2] 朱红霞[2] 滕恩江[2] 

机构地区：[1]扬州市环境监测中心站,江苏扬州225007 [2]中国环境监测总站,北京100012

出　　处：《中国环境监测》2013年第2期65-68,共4页Environmental Monitoring in China

基　　金：国家水专项"水环境质量监测技术方法研究"(2009ZX07527-001)

摘　　要：建立了直接进样-超高效液相色谱分离-紫外吸收测定地表水中丁基黄原酸的分析方法。通过对流动相的优化,确定最佳分析条件:0.050 mol/L超纯水乙酸铵溶液(pH约为9.5)∶乙腈=80∶20等度洗脱,Waters ACQUITY UPLC BEHC18色谱柱(50 mm×2.1 mm,1.7μm)分离,流速0.30 mL/min,进样体积10.0μL,302 nm紫外吸收。结果显示,0.5～20.0μg/L范围线性良好,R=0.9998;2.0、10.0、20.0μg/L重复测定,变异系数小于5%,精密度良好;实际水样加标回收率为95.5%～101.6%;以3倍信噪比计算得出检出限为0.80μg/L。该方法分析速度快,2 min内可完成,满足环境质量监测要求。A rapid analysis method has been developed for butylxanthic acid in surface water by filter-liquid ehromatography-UV absorption. Through optimization of flow, the last analysis conditions were determined. Flow A（0.050 mol/L CH3COONH4, pH in 9.5 ）/flowB（acetonitrile） =80：20, Waters ACQUITY UPLC BEH Cl8（ 50 mm×2.1 mm,1.7 μm）, flow rate 0.30 mL/min, sample volume 10.0 μL, 302 nm UV absorption. Results showed that good linearity was observed in the range of 0. 5 to 20.0 μg/L, R = 0.9998. The RSD was below 5% in three surface water samples were in the range of 95.5% - 101.6%. μg/L. Analysis could be finished in 2 minutes. spiked levels of 2.0,10.0 and 20.0 μg/L. The recoveries of Based on signal to noise ratio, the limit of detection was 0.80 μg/L. Analysis could be finished in 2 minutes.

关 键 词：丁基黄原酸 地表水 直接进样 超高效液相色谱 

分 类 号：X830.2[环境科学与工程—环境工程]