UPLC测定中药材中牡荆苷和异牡荆苷的含量  

Determination of the Contents of Vitexin and Isovitexin in Chinese Medicinal Herbs by UPLC

作　　者：林菲娟[1] 许晓锋[2] 

机构地区：[1]深圳市第二人民医院药剂科,广东深圳518035 [2]广州中医药大学中药学院,广东广州510405

出　　处：《深圳中西医结合杂志》2013年第2期71-74,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:建立同时测定几种药材中牡荆苷和异牡荆苷含量的方法。方法:采用超高效液相色谱(ultra Performance Liquid Chromatography,UPLC)法,以ACQUITY UPLC BEH C18色谱柱(2.1mm×50mm,1.7μm),甲醇-乙腈-0.2%乙酸水为流动相,流速0.3mL·min-1,梯度洗脱,检测波长340nm,柱温35℃,检测时间7min。检测含羞草、小叶榕、布渣叶、蒲葵、软叶刺葵、淡竹叶、木豆叶和白花败酱草等8种中药材中牡荆苷和异牡荆苷的含量。结果:所建立的测定方法符合液相色谱法要求;牡荆苷、异牡荆苷均在5～250ng(r=0.9999)范围内线性关系良好,平均回收率分别为98.40%、98.35%(n=9),RSD分别为0.43%、0.45%。结论:该法快速简便、准确、分离效果好,能同时快速测定这8种药材中牡荆苷和异牡荆苷含量。Objective To establish an reversed phase-high perform ance liquid chromatography（RP-HPLC） method for simultaneous determination of the contents of vitexin and isovitexin in Herbs. Methods The Ultra Performance Liquid Chromatography（UPLC） system consisting of ACQUITYUPLC BEH C18 column （2.1 mm × 50 mm, 1.7μm） and a solution system of methanol-acetonitrile-0.2 % acetic-acid water as the mobile phase with gradient elution was adopted. The flow rate was set at 0.3 mL/min, the detection wavelength was 340 nm and the column temperature was 35℃, each in 7min. Results The linear ranges of vitexin and isovitexin both were 5-250ng （ r = 0.9999）, respectively. The average recoveries （n = 9） were 98.40 % （RSD = 0.43 %）, 97.35 % （RSD = 0.45 %）, respectively. Conclusion The method is quick, simple and accurate with good reproducibility and can be used for determination and quality control of vitexin and isovitexin in Chinese Medicinal Herbs.

关 键 词：牡荆苷 异牡荆苷 UPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]