K562多药耐药细胞株LRP、P-gP、bcl-2基因蛋白表达的观察  

Study of iung resistance protein、P-gP and bcl-2 gene protein expressing in multidrug resistance cell strain of K562

作　　者：沈绍华[1] 叶欣[1] 陈立军[2] 杨惠英[1] 刘爱华[1] 杨宪勇[1] 

机构地区：[1]泰山医学院第一教学医院儿科,山东泰安271000 [2]山东省立医院儿科,山东济南250012

出　　处：《泰山医学院学报》2000年第3期204-206,共3页Journal of Taishan Medical College

摘　　要：目的　探讨LRP(肺耐药相关蛋白 )、P gP(P 糖蛋白 )、bcl 2在K5 6 2多药耐药细胞株中的蛋白表达情况。方法　应用LRP 5 6、P gP(JSB 1)、bcl 2 (10 0 )单克隆抗体 ,通过免疫组化法 ,对K5 6 2细胞株及K5 6 2多药耐药细胞株中LRP、P gP、bcl 2的表达情况进行了测定。结果　K5 6 2细胞株中LRP、P gP、bcl 2表达均阴性 ;而K5 6 2 /VCR耐药细胞株和K5 6 2 /Dox耐药细胞株中LRP、P gP表达阳性 ,bcl 2表达阴性。结论除P gP是产生MDR(多药耐药 )的经典因素外 。Object:To explore the relationship between lung resistance protein (LRP)、P gP 、 bcl 2 expression and multidrug resistance(MDR) in multidrug resistance cell strain of K562.Methods: the K562 cells were cultured by method of increasing selection pressure.The drug resistance of K562/VCR cells to vincristine was 80 times, and K562/Dox cells to doxrubicin was 110 times. LRP, P gP, bcl 2 were detected from K562/VCR and K562/Dox cells by immunohistochemistry using LRP 56,P gP(JSB 1) and bcl 2(100) monclonal antibody. Results: LRP, P gP expression were positive in K562/VCR and K562/Dox cells, bcl 2 negative. Conclusion:LRP expression may be another important facter in MDR of Leukemia.

关 键 词：肺耐药相关蛋白 P-糖蛋白 BCL-2 K562细胞株 

分 类 号：R392[医药卫生—免疫学]