整体填充和颗粒填充β-TCP植骨材料对修复腔隙性骨缺损的影响  

The Effect of β-TCP with Unitary Filling and Particle Filling Methods on Reconstruction of Lacunar Bone Defect of Rabbits

作　　者：王军琳 朱皓东[2] 马许宁[2] 高严鹏[2] 孙龙[2] 吕荣[2] 王军[2] 万海云[2] 

机构地区：[1]第四军医大学口腔医院门诊部,陕西西安710032 [2]第四军医大学西京医院骨科,陕西西安710032

出　　处：《现代生物医学进展》2013年第9期1648-1650,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨不同植骨方法对于β-TCP(β-Tricalcium phosphate,β-TCP)植骨材料修复兔股骨腔隙性骨缺损的效果。方法:24只成年家兔,被随机分为整体填充组(12例)和颗粒填充组(12例)。以膝关节外侧切口入路,在双侧股骨髁部制备6 mm(直径)×10 mm(深度)骨缺损,按照事先随机分组结果,分别进行整体填充植骨和颗粒填充植骨。分别于术后3至6周处死动物,取材标本进行生物力学分析,Micro-CT检测和组织学观察。结果:3周时,整体填充组的最大抗压强度明显优于颗粒填充组(P<0.05),而Micro-CT检测发现新生骨密度(tissue mineral density,TMD)和新生骨体积百分比(bone volume fraction,BV/TV)在两组间均无明显差异,组织学在两组均表现为少量新骨形成;6周时,两组之间的最大抗压强度无明显差异,而Micro-CT检测显示颗粒填充组的TMD和BV/TV均优于整体填充组(P<0.05),组织学上颗粒填充组较整体填充组可见较多的新骨形成。结论:整体填充组提供了更好的生物学强度,颗粒填充组更有利于诱导新骨形成,二者各有优点。Objective： To investigate the effect of β-TCP with unitary filling and particle filling methods on reconstruction of lacunar bone defect of rabbits. Methods： Twenty-four adult rabbits were selected in this study, and were randomly divided into two groups （unitary filling group and particle filling group）. The β-TCP were filled into the cylindrical lacunar bone defect （diameter 6 ram; height 10 mm） in both femoral condyle of the rabbits. At 3 and 6 weeks postoperatively, biomechanics evaluation, micro-CT analysis and histological observation were measured. Results： At the third week, the maximum pressure in unitary filling group was higher than particle filling group （P〈 0.05）, but there were no significant difference between two groups in Micro-CT analysis and the formation of a small amount of,new bone was detected by histological observation in both groups. At 6 weeks, the maximum pressure showed no obvious difference in two groups, however, the Miero-CT analysis indicated that more bone formation were in particle filling group than in unitary filling group （P〈0.05）, and the histological observation showed the same phenomenon. Conclusion： The unitary filling provided better biomechanical performance, and the particle filling benefited the new bone formation, both filling methods had their advantages and disadvantages.

关 键 词：Β-TCP 腔隙性骨缺损 整体填充 颗粒填充 骨修复 

分 类 号：R318.01[医药卫生—生物医学工程] R68[医药卫生—基础医学]