明日叶查尔酮对2型糖尿病大鼠骨骼肌细胞胰岛素抵抗的干预作用(英文)  

Effects of Angelica Keiskei Chalcone on Insulin Resistance of Skeletal Muscle Cells of Type 2 Diabetic Rats

作　　者：赵阳[1] 钟进义[1] 李蕾[2] 尹衍玲[3] 刘贝[1] 

机构地区：[1]青岛大学医学院公共卫生系,山东青岛266021 [2]厦门大学医学院,福建厦门361005 [3]青岛市传染病医院,山东青岛266033

出　　处：《现代生物医学进展》2013年第9期1655-1658,共4页Progress in Modern Biomedicine

基　　金：The youth science research foundation of Shandong Province(QNW001)~~

摘　　要：目的:研究明日叶查尔酮对2型糖尿病大鼠骨骼肌胰岛素抵抗的干预作用。方法:将2型糖尿病大鼠随机分成四组,高、中、低剂量组分别每日经口灌胃给予明日叶查尔酮30、10和5mg/(kg.bw),糖尿病对照组给予等量生理盐水。各组均以高脂饲料喂养。四周后采用葡萄糖氧化酶法检测空腹血糖;放射免疫法检测血清胰岛素含量;免疫组化法检测葡萄糖转运体1和葡萄糖转运体4蛋白表达水平。结果:经图像分析,高剂量组骨骼肌细胞中葡萄糖转运体1和葡萄糖转运体4蛋白表达平均光密度值分别为0.054±0.0064和0.063±0.0139,均较糖尿病对照组显著性升高(P<0.05)。高剂量组空腹血糖和胰岛素水平分别为(12.3±1.64)mmol/L和(25.65±3.34)(μIU/mL),均较糖尿病对照组显著性降低(P<0.05)。结论:明日叶查尔酮可增加2型糖尿病大鼠骨骼肌葡萄糖转运体1和葡萄糖转运体4蛋白表达水平,降低空腹血糖和胰岛素水平,改善胰岛素抵抗状况。Objective： To investigate the effect of angelica keiskei chalcones （AC） on insulin resistance of skeletal muscle cells of type 2 diabetic rats. Methods： Rats with type 2 diabetes were given 30, 10, 5 mg/ （Kg·bw） AC daily by mouth as high, middle and low dose groups respectively. Sailine was given to the diabetic control group by mouth. Four groups were fed with the diet containing high fat. After four weeks, fasting blood glucose was determined by glucose oxidase method. Insulin was assessed by radio-immunity method. The protein expression levels of Glutl and Glut4 in skeletal muscle cells were analyzed by immunohistochemistry method. Results： After analyzed by Image-Pro Plus on immunohistochemistry pictures, the expression levels of Glutl and Glut4 in skeletal muscle cells in high group were 0.054± 0.0064 and 0.063± 0.0139, which were significantly higher than the diabetic control group （P〈0.05）.The levels of fasting blood glucose and insulin in high dose group were （12.3± 1.64） mmol/L and （25.65：1： 3.34） μIU/ml, significantly lower than those of the diabetic control group （P〈0.05）. Conclusion： Angelica keiskei Chalcones may increase the expression levels of Glutl and Glut4 in skeletal muscle cells, decrease fasting blood glucose and insulin of type 2 diabetic rats and improve their insulin resistance condition.

关 键 词：明日叶查尔酮 2型糖尿病 胰岛素抵抗 葡萄糖转运体 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]