中枢反复注射ghrelin对大鼠摄食的影响  

Effects of Administration of Ghrelin into Lateral Cerebral Ventricle on Feeding Dynamic in Rats

作　　者：李莹[1,2] 谷方[1] 杨南[3] 祝海[3] 邱贝贝[1] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理教研室,山东青岛266021 [2]菏泽医学专科学校护理系,山东菏泽274000 [3]青岛市市立医院,山东青岛266011

出　　处：《现代生物医学进展》2013年第11期2005-2009,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30470642;30670780;31071014;81100260;81270460);山东省科技攻关项目(2008GG10002006);山东省卫生厅项目(2007HZ026);青岛市科技局项目(11-2-3-3-(2)-nsh)

摘　　要：目的:Ghrelin是一种主要由胃粘膜产生的28氨基酸肽。因为血浆中的ghrelin完全依赖于摄食获得,所以这种激素对食欲和能量平衡有显著影响。本研究侧脑室注射ghrelin后两小时,通过检测食物容器内剩余食物及水的量,观察中枢应用ghrelin对进食的影响。方法:每天侧脑室注射一次ghrelin(0.4μg),连续注射4天。每天测量体重,同时实验结束后检测腹膜后、附睾脂肪(WAT)含量以及血液中瘦素和胰岛素水平。结果:在第二次侧脑室注射ghrelin后,大鼠第一个和第二个30分钟时间段内进食与对照组相比显著增多(P<0.05),分别为47.2%和63.8%。自第二次注射ghrelin直至治疗结束,大鼠体重显著增加(P<0.05),食物和水的摄入量显著增多(P<0.05)。实验结束后,大鼠腹膜后和附睾WAT含量显著增加(231.4%,P<0.01;84.1%,P<0.05);血清胰岛素水平显著升高(42.3%,P<0.05),而血清瘦素水平显著下降(74.1%,P<0.05)。结论:中枢多次注射ghrelin对自由摄食的成年大鼠摄食具有促进作用。Objective： Ghrelin is 28-amino acid peptide, Which is produced mainly in the stomach. Since plasma ghrelin are strictly dependent on food intake, this hormone has significant effects on appetite and energy balance. The aim of this investigation was to examine the effects of centrally applied ghrelin on feeding dynamism by measuring the amount of food and water in the food container within 2 hours immediately after intracerebroventricular injection of ghrelin. Methods： Four continuous injection of rat ghrelin were administered once per day （0.4 I^g）, into lateral cerebral ventricle （ICV） Body weight was obtained daily, while ending retroperitoneal （RP） and epididymal （EPI） white adipose tissue （WAT） contents as well as blood levels of leptin and insulin were measured. Results： Results showed that in the first and the second 30-min intervals number of approaches to food container were significantly increased already after the 2nd ICV ghrelin application （P〈0.05）, by 47.2% and 63.8% respectively, in comparison with control rats. Centrally applied ghrelin increased body weight after the 2nd injection till the end of treatment （P〈0.05）, which was followed by increased food and water intake （P〈0.05）. At the end of treatment, RP and EPI WAT contents were increased （231.4%, P〈 0.01 and 84.1%, P〈0.05）. Serum insulin levels were elevated （42.3%, P〈0.05） while serum leptin levels were decreased （74.1%, P〈0.05）. Conclusions： These data and the available literature strongly support the opinion that central ghrelin repetitive administration play essential role in food initiation and feeding dynamics in freely feeding adult male rats.

关 键 词：GHRELIN 侧脑室注射 摄食 脂肪组织 

分 类 号：R95-3[医药卫生—药学]