海南岛两株甲病毒基因组3′末端核苷酸序列的克隆与分析  

Cloning and primary analysis of 3'end genome of two alphaviruses isolated from Hainan Province of China

作　　者：赵文忠 周国林[2] 何海怀[2] 付士红[2] 金奇[2] 赵春生 方美玉 蒋廉华 林立辉 梁国栋[2] 侯云德[2] 

机构地区：[1]广州军区医学研究所,广州510507 [2]中国预防医学科学院病毒学研究所病毒基因工程重点实验室

出　　处：《中华实验和临床病毒学杂志》2000年第3期213-217,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金优秀国家重点实验室重点资助课题 !(3982 30 0 2 )

摘　　要：目的　从分子水平鉴定海南省分离的两株甲病毒 (HBb17和M1病毒 )。方法　采用RT-PCR方法 ,分别扩增两株病毒基因组的 3′末端核苷酸序列 ,扩增产物经亚克隆 ,筛选重组子并测定核苷酸序列对序列进行分析。结果　HBb17和M1病毒分别扩增出约 1 6kb和 1 3kb的特异片段。序列分析表明 ,在 3′末端非翻译区HBb17病毒与罗斯河病毒 (国际标准株T48病毒 )核苷酸同源性为99 % ,M1病毒与鹭山病毒 (SAG)同源性为 98% ;两病毒结构基因的E1区序列 ,HBb17病毒与罗斯河病毒核苷酸 (氨基酸 )同源性为 99% (99% ) ,M1病毒与鹭山病毒核苷酸 (氨基酸 )同源性为 97% (99% ) ,M1病毒与盖塔病毒同源性为 94% (98% )。结论　序列分析表明 ,海南岛分离的HBb17病毒属于罗斯河病毒 。objective To further characterize the HBb17 and M1 viruses isolated from Hainan Province by molecular biology.Methods The fragment containing partial E1 and 3' untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned.Recombinant was screened and sequenced.Analysis and comparison were carried out.Results 1.6kb fragment was amplified from HBb17 and a 1.3kb was from M1.Comparison of sequences showed,in 3' untranslated region the nucleotide homology between HBb17 and T48, the prototype of Ross River virus,is 99%,between M1 and Sagiyama virus is 98%. In the partial E1 sequence,the nucleotide(amino acid) homology between HBb17 and T48 is 99%(99%),between M1 and Sagiyama virus is 97%(99%),and between M1 and Getah virus is 94%(98%).Conclusion Sequence analysis showed HBb17 virus belongs to Ross River virus,M1 virus belongs to Sagiyama virus or Getah virus.

关 键 词：甲病毒属 罗斯河病毒 鹭山病毒 克隆 分子生物学 

分 类 号：R373[医药卫生—病原生物学]