抗-HCV阳性无病毒血症患者HCV特异性免疫反应研究  

Study of hepatitis C virus specific immune responses in anti-HCV positive patients without hepatitis C viremia

作　　者：姚鹏[1] 胡学玲[1] 胡大荣[1] 

机构地区：[1]北京军区总医院肝病研究所,北京100700

出　　处：《中华实验和临床病毒学杂志》2000年第3期227-230,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究抗HCV阳性无病毒血症患者特异的体液及细胞免疫状态。方法　对 15例抗HCV阳性无病毒血症患者 ,15例慢性HCV持续感染者及 15例正常对照者进行研究 ,通过MTT、LDH释放法、酶联免疫等方法观察患者外周T细胞反应、自然杀伤 (NK)细胞活性、细胞因子分泌及特异性体液免疫反应。结果　无病毒血症者T细胞增殖反应明显大于持续感染者及对照组 ,并且T细胞对核心区抗原增殖最大 ,NS3次之 ,对NS5则无明显增值。而NK细胞活性较持续感染者及对照组稍高 ,但无统计学差异。此外我们观察了患者抗HCV抗体产生 ,发现无病毒血症患者与持续感染者抗 -HCV抗体产生无明显统计学差异。还观察到在无病毒血症组抗原刺激后产生IFN明显高于持续感染组 ,两组在无抗原刺激下IFN产生水平均极低下。结论　抗HCV阳性无病毒血症患者 ,其T细胞免疫及抗体的产生均高于持续感染者 ,这可能在病毒的清除中起一定作用 ,而免疫反应在慢性HCV感染中的作用及机制 。Objective To study the hepatitis C virus specific immune responses in anti-HCV positive patients without hepatitis C viremia. Methods 15 anti-HCV positive patients without hepatitis C viremia,15 patients with chronic HCV infection and 15 normal controls were selected for this study.The T cell responses,NK cell(natural killer cells)activity Cytokine production and HCV specific antibodies were detected by MTT,LDH release and ELISA. Results Our study showed that the T cell proliferative reaction of patients without hepatitis C viremia was significantly higher than that of patients with chronic HCV infection and normal controls and the T cell response for HCV core antigen were higher than NS3 and NS4,but there was no significant proliferative response to NS5 antigen.We also found that there were no differences in anti-HCV antibody production and NK cell activity between the two groups and the level of IFN-γ in patients without hepatitis C viremia was higher than that in patients with rersistent HCV infection.Conclusion There are a lot of advantageous changes of HCV specific humoral and cellular immune response in anti-HCV positive patients without hepatitis C viremia,these immune responses may play a role in clearance of HCV.

关 键 词：丙型肝炎病毒 细胞免疫 病毒血症 

分 类 号：R392[医药卫生—免疫学]