山东省某些高危人群HIV-1感染者分子流行病学研究  

Molecular epidemiological study on HIV-1 infection in Shandong Province

作　　者：刘传新[1] 邢辉[2] 苏生利[1] 潘品良[2] 黄涛[1] 范秀娟 冯毅[2] 傅继华[1] 赵世立[1] 邵一鸣 

机构地区：[1]山东省卫生防疫站病毒研究室,济南250014 [2]卫生部艾滋病预防与控制中心参比实验室

出　　处：《中华实验和临床病毒学杂志》2000年第3期231-235,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省卫生厅科研基金资助 !(9810 70 10 2 )

摘　　要：目的　了解流行于山东境内HIV - 1毒株的亚型分布及变异情况 ,分析其来源并推测其流行趋势。方法　采集了 2 5份HIV - 1抗体阳性感染者的全血分离单核细胞 (PBMC) ,提取前病毒DNA ,经nested -PCR扩增HIV - 1膜蛋白 (env)基因的C2 -V3区并进行序列测定和亚型分析。结果　 2 5份HIV - 1阳性感染者的PBMC样品中扩增到 2 4份可用于序列测定的HIV - 1env基因片段 ,经序列测定和基因分析鉴别出共有 5种HIV - 1M亚群基因亚型 :A、B、C、E和B′亚型。 13名有偿献血人员均为B′(泰国B)亚型 ,10名回国劳工及 1名回国劳工的女性配偶中存在A、B、C、E四种亚型。结论　山东省HIV - 1各种亚型毒株感染情况复杂 ,因此应加强对献血者及回国劳工等高危人群的检测和控制 ,以防HIV -Objective The aim of this study was to understand the distribution of HIV-1 strain subtypes and their variation in Shandong Province and to analyze their source and predict their epidemic trend.Method We collected the whole blood from 25 anti-HIV-1 antibody positive cases and separated the peripheral blood mononuclear cells.The provirus DNA was extracted and amplified by nested-PCR,after which the HIV-1 membrane protein gene(env) C2-V3 region was sequenced and its subtype was analyzed.Results Among 25 HIV-1 antibody positive cases,24 HIV-1 env gene fragments were amplified and analyzed.5 subtypes of Group HIV-1 M were identified among these cases,that are subtypes A,B,B',C and E.13 occupational blood donors were all classified as subtype B'(Thailand B').A,B,C or E subtypes were discovered in 10 returned laborers working abroad and 1 female spouse of one laborer.Conclusion The condition of HIV-1 subtype infection was complicated in Shandong Province. So,it is important to strengthen the surviellance and control of HIV-1 subtype strain infection in high risk populations especially the occupational blood donors and returned laborers working abroad to prevent its spread in Shandong Province.

关 键 词：HIV-1 膜蛋白质类 序列分析 基因 流行病学 

分 类 号：R181.3[医药卫生—流行病学]