P38MAPK在脂多糖诱导人单核细胞产生肿瘤坏死因子中的作用  

The role of P38 mitogenactivated protein kinase in mediating tumor necrosis factorα production in monocytes stimulated by lipopolysaccharide

作　　者：卢应民 李锦华[2] 顾军 

机构地区：[1]广东普宁华侨医院内科,广东普宁515300 [2]中山医科大学附属第一医院急诊科分子医学研究中心,广东广州510080

出　　处：《中国危重病急救医学》2000年第10期620-623,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金重点基金资助项目!(No.39730140)

摘　　要：目的 :探讨 P38信号通道 (P38MAPK)在脂多糖 (L PS)诱导人单核细胞表达肿瘤坏死因子 (TNFα)中的作用。方法 :采用免疫沉淀法、免疫复合物蛋白激酶测定法、Western blotting检测 P38MAPK在 L PS诱导的人单核细胞产生 TNFα中的激活程度 ,应用逆转录聚合酶链式反应 (RT PCR)和酶联免疫吸附法(EL ISA)观察 P38信号通道抑制剂 SB2 0 35 80对单核细胞 TNFα m RNA和蛋白质表达的影响。结果 :L PS刺激单核细胞引起 P38MAPK激活和 TNFα表达明显增多 ,P38MAPK抑制剂 SB2 0 35 80对单核细胞 TNFα表达有显著抑制作用。结论 :P38MAPK可能是 L PS诱导人单核细胞表达Objective:To investigate the potential role of P38 mitogenactivated protein kinase( P38MA PK) in inducing proinflammatory cytokine 〔tumor necrosis factorα(TNFα)〕 formation in human monocytes stimulated by lipopolysaccharide(LPS).Methods:Immunoprecipitation,immune complex protein kinase assay and Western blotting were used to detect P38MAPK activity in monocytes stimulated by LPS.Reverse transcription polymerase chain reaction (RTPCR) and enzymelinked immunosorbent assay(ELISA) methods were used to investigate the effect of SB203580 (P38MAPK inhibitor) on TNFα mRNA and protein levels in monocytes.Results:LPS caused activation of P38MAPK at time and dosage dependent manner,and SB203580 inhibited not only activation of P38MAPK but also production of TNFα at time and dosage dependent manner.Conclusions:P38MAPK may play an important role in production of TNFα in human monocytes.

关 键 词：P38信号通路 脂多糖 单核细胞 炎症 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]