Cu^(2+)对两种海洋微藻生长和叶绿素荧光特性的影响  

Effects of Cu^(2+) on the Growth and Chlorophyll Fluorescence of Two Species of Marine Microalgae

作　　者：姜恒[1,2] 吴斌 阎冰 吴志强[2] 邢永泽 

机构地区：[1]广西红树林保护重点实验室,广西红树林研究中心,广西北海536000 [2]桂林理工大学,广西桂林541004

出　　处：《广西科学》2013年第1期60-65,共6页Guangxi Sciences

基　　金：广西自然科学基金北部湾重大专项(2011GXNSFE018001);国家海洋公益性行业科研专项(201005012);广西科学研究与技术开发计划项目(桂科合1010019-23);广西科学院科研业务费(11YJ24HS03)资助

摘　　要：运用水样叶绿素荧光仪(WATER-PAM)研究Cu2+胁迫下,湛江叉鞭金藻(Dicrateria zhanjiangensis)和球等鞭金藻3011(Isochrysis galbana MACC/H59)叶绿素荧光特性的变化,同时监测微藻的生长情况。结果就叶绿素荧光参数而言,湛江叉鞭金藻和球等鞭金藻3011的叶绿素荧光参数Fv/Fm,Fv/F0,Yield和ETR均随着Cu2+浓度的增大而明显降低;就生长情况而言,随着Cu2+浓度的增大,两种海洋微藻细胞密度的增长明显变缓。在24h时Cu2+对湛江叉鞭金藻的毒性大于球等鞭金藻3011,48～96h时Cu2+对球等鞭金藻3011的毒性大于湛江叉鞭金藻,Cu2+的毒性随着胁迫时间的延长而逐步增大。The changes of the fluorescence parameters of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 exposed to Cu^2＋ were investigated by means of the chlorophyll fluorescence technology（Water-PAM）,and the growth of these two species of marine microalgae was observed. The results showed that all fluorescence parameters （ Fv/Fm ,Fv/Fo ,Yield and ETR） of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu^2＋ stress. The growth of cell density for Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu^2＋ stress, and it can be expressed as Dicrateria zhanjiangensis Isochrysis galbana MACC/H59 in the 24h while it can be expressed as Isochrysis galbana MACC/H59〉 Dicrateria zhanjiangensis during 48h to 96h. The toxicity of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 increased with the increase of exposure time.

关 键 词：湛江叉鞭藻 球等鞭金藻3011 叶绿素荧光 细胞密度 CU2+ 

分 类 号：X835[环境科学与工程—环境工程]