西藏小型猪IGFBP-3基因甲基化与表达差异分析  

Study on the difference of methylation and expression level of IGFBP-3 gene in Tibetan Mini-pig

作　　者：许无恨[1] 马强[1] 王佳[1] 官员[1] 房希碧[1] 田来明[2] 郝林琳[1] 于浩[1] 张英[1] 刘松财[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]长春市农业科学院,吉林长春130111

出　　处：《扬州大学学报（农业与生命科学版）》2013年第1期7-11,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31072102;31101781)

摘　　要：为研究西藏小型猪类胰岛素生长因子结合蛋白3(IGFBP-3)基因甲基化及表达差异,对IGFBP-3基因的CpG岛甲基化程度、mRNA及蛋白表达水平进行分析,并以军牧一号猪为对照。结果表明:西藏小型猪肝脏组织中IGFBP-3基因CpG岛甲基化水平显著高于军牧一号猪(P<0.05);西藏小型猪不同组织中IGFBP-3mRNA表达量均显著高于军牧一号猪(P<0.05);同时肾脏中IGFBP-3蛋白含量显著高于军牧一号猪(P<0.05)。这为猪生长发育调控及促生长机制提供了分子依据。In order to investigate the difference of the methylation and the expression levels of insulin-like growth factor binding protein 3 （IGFBP-3） between Tibetan Mini-pig and the Junmu-1 white pig, the methaylation of CpG island, the mRNA and the protein expression level of IGFBP-3 were detected in this study. The results showed that： The methyla- tion level of IGFBP-3 gene in the liver of Tibetan Mini-pig was significantly higher than that in the liver of Junmu-1 pig （P〈0. 05）. The mRNA level of IGFBP-3 in the tissues of Tibetan Mini-pig was higher than that in the Junmu-1 pig gen- erally （P〈0.05）, whereas, the same phenomenon of the IGFBP-3 protein expression was only found in the kidney tissue （P〈0. 05）. This would lay certain molecular theoretical basis for the pig resource specific to the growth and development mechanisms of pigs.

关 键 词：西藏小型猪 IGFBP-3基因 甲基化 重亚硫酸盐测序PCR 

分 类 号：S813.24[农业科学—畜牧学]