大豆子叶节再生体系的建立  

Establishment of regeneration system of soybean cotyledonary node

作　　者：刘银[1] 史秀岚[1] 王静磊[1] 刘琪迩[1] 王幼平[1] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2013年第1期68-72,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家转基因生物新品种培育科技重大专项(2009ZX08004-002B)

摘　　要：以大豆子叶节作为外植体,研究不同基因型对丛生芽诱导的影响,筛选出丛生芽诱导率较高的大豆基因型小粒豆(东农50)。在此基础上,以小粒豆的子叶节为外植体,研究种子消毒方法、种子萌发及芽诱导阶段6-苄氨基腺嘌呤(6-BA)对丛生芽诱导的影响以及生根阶段吲哚丁酸(IBA)对生根的影响。在确定氯气消毒法作为适宜大豆种子消毒的方法的基础上,最终确定萌发培养阶段添加1.0mg.L-1 6-BA、芽诱导阶段添加1.67mg.L-1 6-BA、生根培养阶段添加1.0mg.L-1 IBA较宜。In order to establish a cotyledonary node regeneration system for soybean, the cotyledonary nodes of 5 soy- bean genotypes were chosen as explants. Xiaolidou （Dongnong 50）, as one of the genotypes, was screened out with the maximum induction rate of multiple shoots, which was then adopted for the selection of sterilizing methods, 6-BA concen- trations on regeneration of cotyledonary node including germination of seedlings and multiple shoots induction, IBA concentrations on root regeneration. The results indicated that chorine gas seed sterilization would be the best, and seed- lings germinated in the medium with 1.0 mg· L^- 1 6-BA were suitable as explants. The optimal concentration of 6-BA in multiple shoots induction medium was 1.67 mg· L^- 1. The optimal concentration of IBA in root regeneration medium was 1.0 mg· L^- 1.

关 键 词：大豆 子叶节 丛生芽 

分 类 号：S565.1[农业科学—作物学]