牡丹泛素延伸蛋白基因片段克隆与表达分析  

Cloning and expression analysis of the ubiquitin extension protein gene fragment of Paeonia suffruticosa

作　　者：王彦杰[1] 张超[1] 王晓庆[1] 马越[2] 刘爱青 张淑玲 董丽[1] 

机构地区：[1]北京林业大学园林学院/国家花卉工程技术研究中心,北京100083 [2]国家知识产权局专利局专利审查协作北京中心,北京100190 [3]曹州牡丹花木开发总公司,山东菏泽274000 [4]洛阳市花木公司,河南洛阳471000

出　　处：《扬州大学学报（农业与生命科学版）》2013年第1期79-83,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30972030)

摘　　要：采用RT-PCR技术从牡丹(Paeonia suffruticosa)花瓣中克隆得到泛素延伸蛋白基因的cDNA片段,该片段长423bp,编码140个氨基酸残基,命名为PsUBQ,GenBank登录号为JN699053。PsUBQ编码的蛋白是在泛素单体后融合了1个核糖体S27a多肽,经多重序列比对发现该蛋白与拟南芥(Arabidopsis thaliana)、甜樱桃(Prunus avium)、葡萄(Vitis vinifera)等高等植物泛素延伸蛋白的氨基酸序列具有很高的一致性。实时荧光定量PCR分析表明:PsUBQ在牡丹不同组织间表达较稳定,且在切花开放各级别花瓣中呈组成型表达。PsUBQ的克隆与表达为进一步探讨牡丹开放衰老进程中泛素对激素信号的作用机制打下基础,同时为研究牡丹目标基因的表达提供校正标准。A cDNA fragment of ubiquitin extension protein gene was obtained from petals of Paeonia suffruticosa by RT-PCR, which was named PsUBQ and registered in GenBank with accession no. JN699053. This fragment was 423 bp in length and encoded 140 amino acids, which contained a ubiquitin monomer fused with a ribosomal S27a protein. Multi- ple sequence alignment indicated that the PsUBQ protein was highly similar to the homologous proteins of other higher plant species such as ArabidoDsis thaliana, Prunus avium and Vitis vinifera. Real-time PCR analysis showed that PsUBQ was presented in different tissues of Paeonia suffruticosa with stable expression, and constitutively expressed in petals during cut flower opening. The cloning and expression analysis of Paeonia suffruticosa ubiquitin extension protein gene provides a basis for further studying the function of ubiquitin in hormone signaling during flower opening and senes- cence, and also serves as a reference for normalization of target gene expression in Paeonia suffruticosa.

关 键 词：牡丹 泛素延伸蛋白 基因克隆 序列分析 表达 

分 类 号：Q943.2[生物学—植物学]