根际浇灌ABA对冬小麦幼苗抗寒性的影响  

Effect of cold resistance on winter wheat seedlings with ABA pouring around root

作　　者：苍晶[1] 王艳梅[1] 王兴[1] 于晶[1] 王军虹[1] 张达[1] 孙兆猛[1] 樊立华[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2013年第4期36-42,共7页Journal of Northeast Agricultural University

基　　金：东北农业大学创新团队项目(C×2003);东北农业大学科学研究基金(2008);高等学校博士学科点专项科研基金(20112325110003);国家基础科学人才培养基金科研训练项目(J0620001)

摘　　要：为探讨外源ABA调控寒地冬小麦耐受低温胁迫的生理生化机制,试验分别用0、0.1、1、10、100μmol.L-1的ABA对强抗寒冬小麦品种"东农冬麦1号"和弱抗寒品种"济麦22"的三叶期麦苗进行根际浇灌。田间自然降温条件下,分别于日最低温度达0、-10和-25℃时取叶片和分蘖节测定生理生化指标,调查麦苗翌年返青率。结果表明,100μmol.L-1ABA处理显著降低两种小麦叶片和分蘖节中渗透调节物质含量、ABA含量及ABA/GA比值,对东农冬麦1号叶片和分蘖节及济麦22分蘖节GA含量无显著影响,但济麦22叶片中GA含量增加。两品种小麦MDA含量减少,SOD活性显著增加,细胞膜透性增大。10μmol.L-1ABA处理后东农冬麦1号叶片和分蘖节中渗透调节物质含量、ABA含量及ABA/GA比值显著增加,GA含量和MDA含量减少,SOD活性增强。济麦22叶片和分蘖节中可溶性蛋白含量及膜脂过氧化无显著影响,但其叶片和分蘖节可溶性糖含量及分蘖节中ABA含量、ABA/GA比值降低。其他浓度对两种小麦膜脂过氧化及可溶性蛋白含量无显著影响。可见,10μmol.L-1ABA可促进东农冬麦1号抗寒性的提高,而对济麦22效果不显著。In order to investigate the physiological and biochemical mechanism of cold tolerance of winter wheat regulating by exogenous ABA in cold area, two winter wheat cultivars Dongnongdong- mail （cold resistant） and Jimai22（cold susceptible） in 3-leaf stage were poured with ABA （the content were 0, 0.1, 1, 10, 100 μmol- L1） around roots. Under field conditions, leaves and tillering nodes of two winter wheat varieties were sampled when the day lowest temperature was 0, -10,-25 ℃ to measure the physiological indices and to investigate the reviving rate of seedlings. The results showed that contents of osmoregulation substance and ABA, and the ratio of ABNGA in leaves and tillering nodes of two winter wheats were decreased significantly with 100 pmol. L1 ABA. The GA content was not affected notably in tillering nodes of two winter wheat varieties, which was increased in leaves of Jimai22. The MDA content was decreased, the SOD activity was increased and the membrane per-meability rised in all of them. Contents of osmoregulation substance and ABA, and the ratio of ABA/GA in leaves and tillering nodes of the two cultivars were increased significantly with 10 μmol. L1 ABA. Meanwhile, the contents of GA and MDA were decreased and the SOD activity was increased, but soluble protein content and membrane lipid peroxidation were not affected significantly in leaves and tillering nodes of Jimai22. Soluble sugar content in leaves and tillering nodes, and ABA content in tillering nodes, and the ratio of ABA/GA were decreased as well in Jimai22. The other concentrations of ABA did not affected membrane lipid peroxidation and soluble proteins content obviously. The results suggested 10 pmol. L1 ABA could enhance the cold resistance of Dongnongdongmail but not Jimai22.

关 键 词：冬小麦 东农冬麦1号 ABA 抗寒性 

分 类 号：S512[农业科学—作物学]