Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues  

作　　者：WEI Shu LE Tao CHEN Yong XU Jian HE HongQiu NIU XiaoDong LUO JinHua 

机构地区：[1]College of Life Science,Chongqing Normal University [2]Chongqing Academy of Science and Technology [3]Chongqing RuoFeng Bio-Tech Co.,Ltd

出　　处：《Chinese Science Bulletin》2013年第15期1838-1842,共5页

基　　金：supported by the Scientific and Technological Research Project of Chongqing (CSTC2011ggB10009,cstc2012pt-kyys10002,cstc2012cx-rkxB10002);the National Natural Science Foundation of China(81202438);the Natural Science Foundation of the Chongqing Education Committee (KJ110605)

摘　　要：To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable.To quantitatively determine tylosin and tilmicosin in edible animal tissues, a time-resolved fluoroimmunoassay （TRFIA） has been developed and validated. For this purpose, desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates, standards and samples were loaded and, finally, diluted europium-labeled anti-tylosin antibodies were added. Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1. The recoveries were 73.6% to 120.5%, with co- efficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ng g-i There was good correlation （R^2〉0.99） between the TRFIA, an enzyme-linked immunosorbent assay and high performance liquid chromatography data. In conclusion, the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effec- tive and economical method that will enable high-throughput sample screening. The method is expected to be widely applicable.

关 键 词：时间分辨荧光 泰乐菌素 替米考星 动物组织 定量测定 荧光免疫 食用 生物样品 

分 类 号：S859.84[农业科学—临床兽医学]