兔膝关节内纳米超顺磁性氧化铁颗粒标记细胞磁靶向模型建立  

Establishment of a rabbit model of targeted magnetic delivery system of super-paramagnetic iron oxide-labeled chondrocytes for articular cartilage repair

作　　者：王凤玲[1] 林肖[1] 陈加荣[1] 尹力[1] 王富友[1] 杨柳[1] 

机构地区：[1]第三军医大学西南医院关节外科中心,重庆400038

出　　处：《第三军医大学学报》2013年第9期866-869,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30870639)~~

摘　　要：目的建立活体兔膝关节腔内磁靶向细胞移植模型,探讨纳米超顺磁性氧化铁颗粒(superparamagnetic ironoxide,SPIO)标记的种子细胞磁靶向移植修复关节软骨缺损的可行性。方法将8只家兔按随机数字表法分成2组,在其左膝股骨滑车中央制造直径5 mm、深6 mm的圆柱形骨软骨缺损,实验组(n=4)缺损内置入钕铁硼强力磁体,对照组(n=4)置入骨水泥;将体外培养扩增的同种异体兔膝关节软骨细胞经BrdU及SPIO双重标记后注入上述活体兔膝关节腔内,1周后取缺损区组织分别行HE、普鲁士蓝染色及BrdU显色。结果实验动物均存活。HE染色示实验组缺损区内大量细胞聚集生长,SPIO及BrdU双重标记结果显示其来源为外源性注入,形态类似软骨细胞;对照组见缺损区纤维组织增生明显,间以大量炎性细胞,SPIO及BrdU双重标记示外源性注入的细胞散在分布,少量集中于缺损区。实验组与对照组相比,高倍镜下缺损区阳性细胞数有显著性差异(t=6.343,P=0.001)。结论该模型可较好完成SPIO标记的关节软骨细胞活体兔膝关节腔内的磁靶向移植研究,并证实磁靶向技术可以显著提高移植细胞向目标区域聚集生长的效率,且能保留较高的细胞活性。Objective To establish a rabbit model of superparamagnetic iron oxide （SPIO）-labeled chondrocytes magnetic targeting in the treatment of cartilage defects. Methods Eight rabbits were used to create osteochondral defect of 5.0 mm in diameter, 6.0 mm in depth in the left knee joint, and then all animals were divided into 2 groups randomly, that is, experimental group and control. A powerful magnet of neodymium iron boron （NdFeB） was placed into the defect in the experimental group, while the bone cement in the control. Rabbit chondrocytes were labeled with BrdU and SPIO, and then injected into the knee joint cavity of rabbit models. Animals were sacrificed in 1 week after the injection. The distal femurs containing the defects were resected, fixed, decalcified, and then performed by HE, Prussian blue and anti-BrdU staining. Results All animals were alive at the end of the experiment. Histological and immunohistochemical staining showed that a large of double-labeled chondrocytes were deposited around the osteochondral defect area, and the double-labeling indicated these cells were exogenous and in a shape like chondrocytes. But in the control group, fibrotic proliferation was observed in the defect, with a great deal of inflammatory cells and some scattered double-labeled cells （ a few deposited in the middle of defect）. Significant difference was found in the number of double positive cells in the defect between the 2 groups （t = 6. 343, P = 0. 001 ）. Conclusion This animal model displayed that BrdU and SPIO-labeled chondrocytes can be targeted to the bone defect of rabbit knee joint through magnetic targeting transplantation, indicating magnetic targeting improves transplanted cells＇ alive and growth in the target place. It can be applied for the research of magnet targeting in the repair of osteochondral defect in rabbits.

关 键 词：磁靶向 SPIO 骨软骨组织工程 关节软骨缺损 

分 类 号：R332[医药卫生—人体生理学] R329.28[医药卫生—基础医学]