蛇床子素与DNA的相互作用研究  

Study on the interaction of Osthole and Deoxyribonucleic acid

作　　者：张红艳[1] 邹艳辉[1] 余宇燕[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国医药科学》2013年第7期33-34,共2页China Medicine And Pharmacy

基　　金：国家自然科学基金项目(81202914);福建省卫生厅青年科研基金(2012-2-61)

摘　　要：用光谱法研究了蛇床子素与脱氧核糖核酸的相互作用,结果表明,在最大发射波长408nm处,脱氧核糖核酸能显著地猝灭蛇床子素的荧光信号。在pH=5.5时,信号猝灭的强度△F最大,并且体系的荧光强度与DNA浓度在10～40nmol/L范围内呈良好的线性关系。The interaction of the Osthole and Deoxyribonucleic acid was studied by spectrometric methods. The results showed that the interaction caused the decrease of fluorescence intensity of Osthole at the maximum emission wavelength of 408 nm. Moreover, the decreased value A F reached the maximum at pH=5.5. It showed that the value of F varied linearly with the concentration of Deoxyribonucleic acid over the range from 10 nmol/L to 40 nmol/L under the optimal conditions.

关 键 词：蛇床子素 脱氧核糖核酸 相互作用 

分 类 号：R285[医药卫生—中药学]