西藏药用植物螃蟹甲可培养内生真菌的分离、鉴定及抗HIV-1整合酶链转移活性研究  

Isolation,idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii Mukerjee

作　　者：张大为[1] 赵明明[1] 陈娟[1] 李超[1] 郭顺星[1] 

机构地区：[1]中国医学科学院、北京协和医学院药用植物研究所,北京100193

出　　处：《药学学报》2013年第5期780-789,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31170016,81202438)

摘　　要：从西藏药用植物螃蟹甲(Phlomis younghusbandii Mukerjee)的叶和茎中分离得到52株内生真菌。经分子鉴定(基于ITS序列和28S序列),内生真菌分属于Phoma、Chaetosphaeronema、Fusarium、Leptosphaeria等12属,其中拟茎点霉属(Phoma)为其优势菌群。使用原核表达的可溶型一型人类免疫缺陷病毒(humanimmunodeficiency virus type 1,HIV-1)的整合酶(integrase,IN),对52株内生真菌的发酵产物进行体外抗HIV-1整合酶链转移反应活性试验,结果发现,来自Chaetosphaeronema属真菌的7个样品对整合酶链转移反应表现出强烈的抑制作用,其对应的IC50值分别为6.60、5.20、2.86、7.86、4.47、4.56和3.23μg·mL-1。该研究表明,螃蟹甲内生真菌在发现抗HIV-1整合酶活性物质方面有进一步的开发价值。A total of 52 endophytic fungi were isolated from roots and stems of Tibetan medicinal plant Phlomis younghusbandii Mukerjee. These fungal isolates were molecularly identified based on ITS sequnces and 28S sequences distributed to 12 genera, including Phoma, Chaetosphaeronema, Fusarium and Leptosphaeria, etc. Among them, the dominant genus was Phoma. Extracts of all strains were evaluated for anti-HIV-1 integrase activity by using soluable integrase expressed in E. coli BL21 （DE3）. The results showed that seven samples from five fungal endophytes PHY-24, PHY-38, PHY-40, PHY-51, PHY-53, which belonged to genus Chaetosphaeronema, inhibited strand transfer reaction catalyzed by HIV-1 integrase with ICs0 values of 6.60, 5.20, 2.86, 7.86, 4.47, 4.56 and 3.23 μg.mL-l respectively. In conclusion, the endophytic fungi of Phlomis younghusbandii Mukerjee are valuable for further screening anti-HIV-1 integrase agents.

关 键 词：螃蟹甲 内生真菌 HIV-1 整合酶 抑制剂筛选 

分 类 号：R931[医药卫生—生药学]