MicroRNA-29抑制胰岛素刺激的大鼠L6细胞葡萄糖吸收  

MicroRNA-29 suppresses glucose uptake stimulated by insulin in L6 myotube cells

作　　者：刘长征[1] 李静静[2] 焦涛[1] 何小东[2] 常永生[1] 

机构地区：[1]中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京100005 [2]中国医学科学院北京协和医院基本外科,北京100730

出　　处：《基础医学与临床》2013年第5期562-566,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金青年科学基金(81100608)

摘　　要：目的研究microRNA-29(miR-29)在糖尿病模型大鼠(GK)骨骼肌组织中的表达,并探讨其对大鼠骨骼肌细胞L6葡萄糖吸收的影响。方法利用real-time PCR方法检测miR-29家族,miR-29a、miR-29b及miR-29c在GK大鼠骨骼肌组织中的表达特征。L6骨骼肌细胞诱导分化为肌管细胞,经葡萄糖与胰岛素处理后,利用real-time PCR与Northern blot分析miR-29家族的表达情况。选择腺病毒表达系统在L6分化的肌管细胞中过表达或抑制内源miR-29的功能,利用葡萄糖吸收实验检测胰岛素刺激的葡萄糖吸收情况。结果 miR-29a、miR-29b及miR-29c在GK大鼠骨骼肌组织中表达上调。L6分化的肌管细胞经葡萄糖与胰岛素处理,miR-29b和miR-29c的表达上调,而miR-29a表达无明显变化。在L6分化的肌管细胞中过表达miR-29可以明显抑制胰岛素刺激的葡萄糖吸收。结论miR-29参与了GK大鼠骨骼肌细胞葡萄糖耐受及胰岛素抵抗的产生,抑制其表达可能是治疗Ⅱ型糖尿病的一种新策略。Objective To evaluate the expression profile of miR-29 family in skeleten muscle of GK rats and to fur- ther probe the function of miR-29 family in glucose uptake in L6 cells induced by insulin. Methods The expres- sion of miR-29 family was detected by RT-PCR. miR-29 level in L6 cells treated by glucose and insulin was meas- ured by using real-time PCR and Northern Blot. Gain or loss of function of miR-29 was achieved by a recombinant adenovirus system and the effect of miR-29 on insulin resistance was measured by insulin-induced glucose uptake assay. Results miR-29a, miR-29b, and miR-29c expression was upregulated in the skeletal muscle of GK rats and miR-29b and miR-29c expression levels were elevated in L6 cells treated by glucose and insulin. Moreover, enforced expression of miR-29 significantly suppressed insulin-induced glucose uptake in L6 cells and loss-of-func- tion of miR-29 by using Ad-sp-miR-29 led to an increase of glucose uptake. Conclusions miR-29 family is involved in the glucose tolerance and insulin resistance of GK rats and targeting miR - 2 9 maybe provide a novel strategy for the treatment of type Ⅱdiabetes.

关 键 词：microRNA-29 Ⅱ型糖尿病 葡萄糖耐受 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]