强脉冲光对豚鼠糖皮质激素所致皮炎及皮肤萎缩的治疗机制  

The Effect of Intense Pulesd Light on Corticosteriod induced Dermatitis and Skin Atropy in Guinea Pigs

作　　者：王燕[1] 马琼[1] 孙素姣[1] 段静娴[1] 陈丽华 

机构地区：[1]云南省大理学院附属医院美容科,云南大理671000

出　　处：《中国皮肤性病学杂志》2013年第5期445-448,共4页The Chinese Journal of Dermatovenereology

基　　金：大理学院青年教师科研基金(KYQN2009-12)

摘　　要：目的研究强脉冲光(intense pulsed light IPL)对豚鼠糖皮质激素所致皮炎及皮肤萎缩的治疗机制,为临床上治疗该病提供实验依据。方法将15只雌性豚鼠均分为A,B,C三组,A,B组背部左右两侧外擦皮康王霜制成动物模型,左侧为IPL照光区,共照射2次,间隔为3周。C组不作任何处理。A组于初次照射后8天、B和C组于第8周取受试部位皮肤行HE染色,观察各组皮肤病理变化。结果 A组照光区真皮内淋巴细胞计数较对照侧明显减少,差异有统计学意义(P<0.01)),同时扩张的毛细血管也明显减少;B组照光区表皮层数略增加,真皮厚度明显增厚,与对照侧比较,差异有统计学意义(P<0.05),且照光区真皮浅层见较多成纤维细胞,胶原纤维排列整齐、致密,染色均一。结论强脉冲光对豚鼠糖皮质激素所致皮炎的血管扩张、淋巴细胞浸润有改善作用,可促进表皮层数增加,刺激胶原产生,增加真皮层厚度。Objective To research the mechanisms of intense pulsed light on corticosteriod-induced dermatitis and skin atropy in guinea pig, and provide laboratory data for treatment of the problem. Methods Fifteen female guinea pigs were divided into A, B, C groups. Compound miconazole nitrate cream were applied to the both sides of the back in A, B groups, and the animal models were completed. The C group were normal control. Then, the left sides were irradiated by IPL at the internals of 3 weeks for ltimes,2 times in totah At last,the skin of both sides of guinea pigs were given eosin staining, A group at the first 8 days after irradiation while the B and C groups at the end of 8 weeks,the changes of the histopathology were observed. Results In A group, the lym-phocyte count was significantly lower in IPL-treated-side than that in the positive control side（P 〈 0.01 ）, and regression of telangietasis in IPL-treated-side . In B group, the increase in the layer number of keratinocytes and the thickness of dermis were significantly thickner in IPL-treated-side than that in the positive control side（P 〈 0.05, P 〈0. 01 ） ,the arrangement of collagen fibers were compact and orderly in IPL-treated-side. Conclusion IPL could improve the espansive caplliaries and infiltration of lymphocytes effectively, and enhance the quan- tity of collagen, increase the layer of keratinocytes and the thickness of dermis in corticosteriod-induced derma-titis and skin atropy.

关 键 词：强脉冲光 糖皮质激素 皮炎 皮肤萎缩 豚鼠 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]