氨基酮戊酸光动力治疗尖锐湿疣局部免疫反应的研究  

Local immune response in condyloma acuminatum treated with aminolevulinic acid-photodynamic therapy

作　　者：张云凤 缪飞 王宏伟[2] 吕婷[2] 李晶晶 王秀丽 

机构地区：[1]上海市皮肤病医院,上海市200050 [2]复旦大学附属华东医院

出　　处：《中华皮肤科杂志》2013年第5期309-312,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81272990）;上海市卫生局科研课题计划（20124034）

摘　　要：目的探讨氨基酮戊酸光动力（ALA—PDT）治疗尖锐湿疣的局部免疫反应。方法分别采用体外细胞共培养体系和在体免疫组化方法进行研究。将前期建立的稳定表达人乳头瘤病毒16E7蛋白HaCaT细胞株即HPVl6E7／HaCaT细胞作为HPV感染角质形成细胞模型，将ALA—PDT处理后的HPVl6E7／HaCaT细胞与外周血单一核细胞（PBMC）经Transwell小室共培养，3h后观察PBMC的趋化情况；同时采用免疫组化方法研究10例尖锐湿疣患者光动力前及光动力后1、2、3、48h时，皮损中CD4＋T细胞、CD8＋T细胞、CD68＋巨噬细胞的数量及CD47CD8＋T细胞数比值变化情况。结果与ALA—PDT处理后的HPVl6E7／HaCaT细胞共培养3h后，PBMC有明显趋化现象。临床标本免疫组化结果显示，48h时CD4T细胞、CD8＋T细胞数量及其比值明显升高（P〈0．05），而CD68＋巨噬细胞在3h及48h均升高（P〈0．05）。结论ALA—PDT治疗尖锐湿疣的过程中，可诱导机体产生局部抗病毒免疫反应。Objective To investigate the local immune response in condyloma acuminatum treated with aminolevulinic acid-photodynamic therapy （ALA-PDT）. Methods In vitro and in vivo studies were performed. A previously established keratinocyte eel1 line human papilloma virus （HPV） 16E7/HaCaT which stably expresses HPV16E7 protein was used in this study. Peripheral blood mononuclear cells （PBMCs） were separated from 10 healthy volunteers. After pretreatment with ALA-PDT, HPV16E7/HaCaT cells were cocultured with the PBMCs for 3 hours in a Transwell chamber followed by the observation of chemotactic migration of PBMCs. Tissue samples were obtained from the lesions of 10 patients with condyloma acuminatum before, and at 1, 2, 3 and 48 hours after the first session of ALA-PDT. lmmunohistochemistry was conducted to determine the number of CD4＋ T cells, CD8＋ T cells and CD68＋ macrophages as well as CD4/CD8 T-cell ratio in the tissue samples. Results After 3-hour cocuhure with HPV16E7/HaCaT cells pretreated by ALA-PDT, PBMCs showed apparent chemotactic migration. Immunohistoehemistry revealed a statistical increase in the number of CD4＋ T ceils , CD8＋ T cells and CD4/CD8 T-cell ratio at 48 hours （all P 〈 0.05） ,as well as in the number of CD68＋ macrophages at 3 hours and 48 hours （both P 〈 0.05） after the first session of ALA-PDT. Conclusion ALA-PDT may induce local antiviral immune response in condyloma acuminatum.

关 键 词：尖锐湿疣 氨基酮戊酸 光化学疗法 免疫 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]