温和灸对高脂血症大鼠血脂及血清NO的影响  

Effects of mild moxibustion on contents of blood lipoids and serum NO in hyperlipidemia rats

作　　者：张会芳[1] 王玲玲[1] 嵇明月[1] 周小云[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《中国针灸》2013年第5期438-442,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973计划)项目:2009CB 522905

摘　　要：目的:观察温和灸治疗高脂血症的效应机制。方法:将60只SD大鼠随机分为5组,正常组、模型组、艾灸组、针刺组、药物组,每组12只,后4组用自制脂肪乳剂灌胃4周,建立大鼠高脂血症模型,正常组给予0.9%氯化钠溶液。造模成功后开始治疗,正常组、模型组不做治疗,艾灸组、针刺组选用"足三里""神阙"穴,分别用艾灸、针刺进行治疗,每日1次;药物组给予洛伐他汀灌胃,10mg/kg,每日1次。治疗期间模型组、艾灸组、针刺组、药物组继续脂肪乳剂灌胃,治疗4周结束。采用生化比色法测定血脂4项,用硝酸还原酶法检测血清一氧化氮(NO)的含量。结果:与模型组比较,艾灸组、针刺组、药物组血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)及NO均降低(P<0.01,P<0.001),同时还有升高高密度脂蛋白胆固醇(HDL-C)的趋势,但差异无统计学意义。艾灸组降低TC、LDL-C、NO的效果明显优于药物组(P<0.01,P<0.05)。艾灸组NO与TC、LDL-C呈正相关(P<0.05,P<0.01),药物组NO与TC、LDL-C呈负相关(均P<0.01)。结论:温和灸对高脂血症大鼠血脂及血清NO都具有良性调节作用,温和灸可以通过降低大鼠血清NO发挥其抗血管损伤、抗炎性反应、预防动脉粥样硬化的作用。Objective To observe the effect mechanisms of mild moxibustion in treatment of hyperlipidemia.Methods Sixty SD rats were randomly divided into a normal group, a model group, a moxibustion group, an acupuncture group and a medication group, 12 rats in each group. The hyperlipidemia model was established by introgastric administration of fat emulsion for 4 weeks in the later four groups, and 0.9 % sodium chloride was given in the normal group. Treatments started when hyperlipidemia model was sullessfully established. No treatment was given in the normal group and the model group. The acupuncture group and the moxibustion group were treated with acupuncture and moxibustion at Zusanli （ST 36） and Shenque （CV 8）, respectively, once a day. The medication group was treated with introgastric administration of lovastatin （10 mg/kg）, once a day. The model group, acupuncture group, moxibustion group and medication group were treated with continuing introgastric administration during the 4-week treatment. Four blood lipoids items were detected by biochemical colorimetry, and the contents of serum NO were measured by nitrate reductase method. Results Compared with the model group, the con- tents of serum total cholesterol （TC）, triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C） and NO in the moxibustion group, acupuncture group and medication group were decreased （P〈0.01,P〈0. 001）, meanwhile, high-density lipoprotein cholesterol （HDL-C） showed a increasing trend without statistical significance. The moxibustion group was better than the medication group at the effect in decreasing the contents of TC, LDL- C and NO. NO had positive correlation with TC and LDL-C in the moxibustion group （P〈0.05, P〈0.01）, while NO had negative correlation with TC and LDL-C in the medication group （both P〈0.01）. Conclusion Mild moxibustion has a favorable regulative action on blood lipoids and serum NO in hyperlipidemia rats. Mild moxibustion can play a role in anti-vascular injury, an

关 键 词：高脂血症 温和灸 一氧化氮 血管内皮功能障碍 

分 类 号：R245.8[医药卫生—针灸推拿学]