妊娠肝内胆汁淤积症患者胎盘促肾上腺皮质激素释放激素受体CRHR-1的表达  

Expression of corticotrapin-releasing hormone receptor type-1 in intrahepatic cholestatic placental tissue

作　　者：刘正飞[1] 何毛毛[1] 周凡[1] 高冰心[1] 王晓东[1] 

机构地区：[1]四川大学华西第二医院妇产科妇儿疾病与出生缺陷教育部重点实验室,成都610041

出　　处：《中华肝脏病杂志》2013年第5期381-384,共4页Chinese Journal of Hepatology

基　　金：长江学者和创新团队发展计划（IRT0935）;四川省科技支撑计划项目（2009SZ0016）

摘　　要：目的观察妊娠肝内胆汁淤积症（ICP）胎盘CRHR-1的表达情况。方法随机选择于37～40周择期剖宫产终止妊娠的ICP与正常妊娠各10例，采用Westemblot及巢式实时荧光定量PeR测定胎盘CRHR-1表达强度。正态分布数据以均数土标准差-X±S）表示，采用t检验。偏态分布数据采用中位数（M）及四分位距（IQR）表示为M（IQR），采用Wflcoxon秩和检验。检验水准a（双侧）=0．05。结果CRHR-1cDNA目的扩增片段为172bp。ICP组胎盘CRHR-1表达的免疫杂交蛋白条带荧光强度1．55±0．28弱于正常组1．60±0．37，但两组比较，差异无统计学意义（t=0．349，P=0．732）l巢式实时荧光定量PeR扩增CRHR-1mRNA相对含量0．139（0．268）高于正常组的0．031（0．245），两组比较，t=1．504，P=0．136，差异无统计学意义。结论ICP胎盘CRHR-1有低表达趋势，可能导致ICP胎盘小叶绒毛血管容积下降和CRH超短阳性反馈回路受损。Objective To explore the expression level of corticotropin-releasing hormone receptor type-1 （CRHR-1） in intrahepatic cholestatic placental （ICP） tissue. Methods Human placental samples were collected from 10 ICP patients and 10 healthy controls after parturition at 37-40 weeks of gestation. CRHR-1 protein and mRNA expression was assessed by western blotting and nested-real-time fluorescence quantitative PCR, respectively. Normally distributed data were summarized as mean ＋ standard deviation, and intergroup comparisons were made by two-tailed Student＇s t-test. Non-normally distributed data were presented as median with interquartile range, and intergroup comparisons were made by Wilcoxon test. For all statistical analyses, a two-tailed P-value of 〈 0.05 was considered statistically significant. Results The CR/-IR-1 fluorescence intensity was lower in ICP tissues （1.55 ± 0.28） than in placental tissues from healthy controls （1.60 ± 0.37）, but the difference did not reach statistical significance （t = 0.349, P = 0.732）. The CRHR-1 mRNA content was slightly higher in the ICP tissues [0.139（0.268）] than in the placental tissues from healthy controls [0.031（0.245）], but the difference did not reach statistical significance （t = 1.504, P = 0.136）. Conclusion CRHR-1 expression is decreased in ICP tissues, which may lead to a smaller volume of placental lobular villi vessels and restrict the CRH positive feedback loop, ultimately promoting acute hypoxic stress and possible harm to the fetus.

关 键 词：妊娠 胆汁淤积 肝内 胎盘 促肾上腺皮质激素释放激素受体 

分 类 号：R714.2[医药卫生—妇产科学]