姜黄素对Raji细胞凋亡及bFGF表达影响的观察  

Effect of curcumin on apoptosis and bFGF expression in lymphoma Raji cells

作　　者：朱红波[1] 周丽[1] 

机构地区：[1]泰山医学院附属医院血液内科,山东泰安271000

出　　处：《中华肿瘤防治杂志》2013年第8期596-599,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨抗血管生成药物姜黄素对人非霍奇金淋巴瘤(NHL)Raji细胞凋亡及碱性成纤维细胞生长因子(bF-GF)表达的影响,为临床抗血管新生治疗NHL提供理论依据。方法:不同浓度(5、10、25和50μmol/L)姜黄素作用Raji细胞不同时间后,倒置相差显微镜下观察Raji细胞形态及生长情况;Annexin V-FITC双标流式细胞术检测姜黄素对细胞凋亡的作用;ELISA检测姜黄素对Raji细胞上清中bFGF含量的影响。结果:显微镜下可见,随姜黄素浓度的增加,Raji细胞由成团生长变为单个散在,细胞数明显减少,细胞体积变小,折光性减弱,有的细胞膜破裂,可见细胞碎片;流式细胞术检测结果显示,作用24、48和72h后10μmol/L姜黄素组细胞凋亡率分别为(11.12±1.05)%、(14.74±0.46)%和(25.11±1.43)%,25μmol/L姜黄素组分别为(25.07±5.40)%、(33.50±5.89)%和(55.98±7.41)%,均高于对照组凋亡率(1.75±0.28)%、(2.25±0.29)%和(2.77±0.61)%,差异有统计学意义,P<0.01。ELISA法检测结果显示,作用24、48和72h对照组上清液中bFGF浓度分别为(643.41±57.12)、(732.26±60.19)和(931.27±81.04)ρg/mL;10μmol/L姜黄素处理组分别为(212.76±20.07)、(173.23±14.76)和(107.21±12.37)ρg/mL;25μmol/L姜黄素处理组分别为(95.32±11.59)、(71.24±10.03)和(36.41±7.58)ρg/mL;Raji细胞经10、25μmol/L姜黄素作用24、48和72h后,bFGF表达水平明显低于对照组,在3个时间点的表达量与对照组相比,差异有统计学意义,P<0.01;不同浓度姜黄素处理组上清液中bFGF含量差异亦有统计学意义,P<0.05。结论:姜黄素对Raji细胞凋亡具有促进作用,能够抑制Raji细胞分泌bFGF,其抑制作用具有浓度、时间依赖性,提示姜黄素可能有抑制血管新生的作用。OBJECTIVE：To detect the effects of curcumin on apoptosis and bFGFs＇expression in Raji cells,and provide a theory evidence for therapy of anti-vessel neogenesis. METHODS： After Raji cells were treated with curcumin at different concentrations and different time,the morphological changes of cells were observed by inverted phase contrast microscope,and the cell apoptosis was tested by flow cytometry; the change of bFGF contents in cultured supernatants of Raji cells treated with different concentratins of curcumin were measured by ELISA. RESULTS：The morphological changes ot＂ cells were observed by inverted phase contrast microscope, the Raji cells turned into disorder from conglobation, the cell population obviously was decreased and the cell volume shrinked, the refraction weakened, the cell membrance disrupted,cell debris existed. The apoptosis rate of Raji cells was increased as the concentration of curcumin and action time were increased. When curcumin concentrations were 10 and 25 μmol/L, the apoptosis rate of Raji cells were （l11. 12 ±1.05）%,（25.07±5.40）% after 24 h,（14.74±0.46）%,（33.50±5.89）% after 48 h and （25. 11±1.43）%,（55.98± 7.41） % after 72 h,which were higher than that of control group E（1.75±0.28）%, （2.25±0.29）%,（2.77±0.61）%]and there was a significant difference （P〈0.01）. The change of bFGF contents in cultured supernatants of Raji cells treated with different concentratins of eurcumin（ 10,25 μmol/L） ,in different time（24,48,72 h） were measured by ELISA, the 10μlmol/L curcumin group levels of bFGF were （212.76 ± 20.07）, （173.23 ± 14.76） and （107.21±12.37） ρg/mL; the 25 μmol/L curcumin group levels of bFGF were （95.32±11.59）, （71.24±10.03） and （36.41 ±7.58） ρg/mL,significantly lower than that of the control groups （643.41~ 57.12）, （732.26±60. 19） and （931.27±81.04） pg/mL. CONCLUSIONS..Curcumin can promote the Raji cells apoptosis,inhibit bFGF secretion of Raji cells and this /＇uncti

关 键 词：姜黄素 药理学 流式细胞术 淋巴瘤 非霍奇金 细胞凋亡 药物作用 

分 类 号：R285.5[医药卫生—中药学] R733.1[医药卫生—中医学]