葛根素抑制大鼠离体胃肠平滑肌收缩活动及机制探讨  

Inhibitory effects of puerarin on contractile activity of isolated gastrointestinal smooth muscle in rats and the related mechanisms

作　　者：张立雪[1,2] 李红芳[1,2,3] 金姗[1] 汪龙德[4] 马勤[1,2] 都中蕊[1,2] 黄金炳[1,2] 

机构地区：[1]兰州大学基础医学院生理学教研室 [2]甘肃省新药临床前研究重点实验室 [3]兰州大学基础医学院机能实验室 [4]甘肃中医学院附属医院

出　　处：《中国临床药理学与治疗学》2013年第4期394-398,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省科技厅自然科学基金项目(1010RJZA111);甘肃省中医药科研项目(GZK-2010-38);兰州市科技局社会发展项目(医药卫生)(2010-1-88)

摘　　要：目的:观察葛根素对大鼠离体胃肠平滑肌收缩活动的影响,并探讨其作用机制。方法:取大鼠胃肠平滑肌条,安置在恒温灌流肌槽中,记录胃肠平滑肌条的收缩活动。结果:葛根素能降低胃肠平滑肌条的张力,减小其收缩波平均振幅,并有剂量依赖关系,以胃体收缩幅度为指标,用Lo-gistic法计算IC50为82.590μmol/L(r=0.498)。酚妥拉明、L-NG-硝基精氨酸和格列本脲可部分阻断葛根素对大鼠胃体收缩活动的抑制作用,用Logistic法计算IC50分别为222.408μmol/L(r=0.495)、166.945μmol/L(r=0.446)、149.902μmol/L(r=0.596)。结论:葛根素对大鼠胃肠平滑肌条收缩活动具有抑制作用,这种抑制作用部分经由肾上腺素能α受体、NO途径及KATP通道所介导。AIM： To investigate the effects of Puerarin （Pue） on contractile activity of Gastro- intestinal smooth muscle of rats and to explore its underlying mechanisms. METHODS： Gastro- intestinal smooth muscle strips were taken out from rats. Each isolated muscle strip was put in a tissue chamber contains 5 mL Krebs solution and the motility of muscle strips was recorded simultaneously. RESULTS： Puerarin could re- duce the resting tension and mean contractile amplitude of gastrointestinal smooth muscle strips, which were dose dependent. To gastric body contraction amplitude for index, with Lo- gistic method for calculating IC50 was 82. 590 μmol/L （r =0.498）. Phentolamine, L-NG-ni-troarginine, Glibenclamide partially blocked the inhibition of puerarin on the contractile activity of the rat gastric body. IC50 was 222. 408 μmol/ L（r=0.495）, 166.945 μmol/L（r=0.446）, 149. 902 μmol/L （ r = 0. 596 ）, respectively. CONCLUSION. Puerarin has inhibitory action on the isolated gastrointestinal smooth muscle strips of rats, which is partially mediated via ad- renergic a receptor and related to NO pathway and potassium channel.

关 键 词：葛根素 胃肠平滑肌 酚妥拉明 左旋- NG-硝基精氨酸 格列本脲 

分 类 号：R965.2[医药卫生—药理学]