慢病毒介导的RNA干扰技术在滤过术后抗瘢痕的研究  

Application of lentivirus-mediated RNA interference technology in inhibiting cicatrization after glaucoma filtering surgery

作　　者：席文群[1,2] 成洪波[1,2] 张国明[1,2] 席兴华[1,2] 曹玉丽[1,2] 

机构地区：[1]暨南大学附属第二临床学院 [2]深圳市眼科医院,广东省深圳市518031

出　　处：《眼科新进展》2013年第5期489-492,共4页Recent Advances in Ophthalmology

基　　金：深圳市科技计划项目(编号:201002132)~~

摘　　要：青光眼滤过术后眼压失控、手术失败的主要原因是滤过泡瘢痕化。转化生长因子-β是瘢痕形成的重要因素。因此,术后抗瘢痕形成的关键是抵抗以转化生长因子-β为主的参与瘢痕形成的细胞因子。RNA干扰是一项高效、特异的调节基因表达的技术,现已成为研究基因功能和信号转导的有力工具。随着对疾病分子机制认识的加深及生物技术的发展,慢病毒介导的RNA干扰具有高效而稳定的基因转移效率,比其他逆转录病毒载体更适合于体内试验及临床研究。After glaucoma filtering surgery, intraocular pressure get out of control and surgery fail mainly because of filtering bleb cicatrization, to which transforming growth factor-β（TGF-β） contributes enormously. The key to inhibiting cicatrization postoperatively is reducing cell factors participating cicatrization, especially TGF-β. RNA interference technology can regulate the expression of gene efficiently and specifically, which has become a powerful tool to study gene function and signal transduction. With a better understanding of the molecular mechanism of diseases and the development of biological technology, lentivirus-mediated RNA interference technology today is safe and stable in gene transferring. It is more suitable for in vivo tests and clinical studies than the other retroviruses.

关 键 词：青光眼滤过术 转化生长因子 瘢痕形成 慢病毒 RNA干扰 

分 类 号：R512.62[医药卫生—内科学]