Kozak序列对金黄色葡萄球菌黏附因子FnBPA-A DNA疫苗诱导小鼠免疫应答的影响  

Effect of Kozak sequence on mice DNA vaccine immunization of Staphylococcus aureus adhesion fibronectin-binding protein FnBPA-A

作　　者：苏艳[1] 王世民[1] 邵俊高[1] 张宝江[1] 韦海娜[1] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《生物工程学报》2013年第4期458-465,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.31160191;31260222);新疆维吾尔自治区普通高校重点学科基础兽医学科项目资助~~

摘　　要：黏附因子FnbpA(纤连蛋白结合蛋白A,Fibronectin binding protein A)是金黄色葡萄球菌表面的蛋白质成分,是该菌感染早期最重要的致病因子,可促进其对寄主组织的侵入,也是一个有潜力的免疫靶标。将牛乳源金黄色葡萄球菌中FnBPA基因的A功能区克隆至真核表达载体中,分别构建含Kozak序列和不含Kozak序列的FnBPA-A基因真核表达载体,重组质粒经鉴定测序正确后,免疫C57BL/6小鼠检测其抗体水平和淋巴细胞增殖情况,并对各组小鼠进行攻毒实验。检测的结果表明Kozak修饰的重组DNA在血清抗体效价(P<0.05)和免疫保护率方面均优于不含Kozak序列的重组DNA,在刺激淋巴细胞增殖方面Kozak修饰的重组DNA的刺激效果虽然也高于不经修饰的重组DNA,但是差异不显著(P>0.05)。根据总体的免疫效果来看,Kozak序列对增强FnBPA的重组DNA疫苗诱导的免疫应答起了不容忽视的作用。Fibronectin-binding protein （FnBPA） is a protein that expresses on cell surface of Staphylococcus aureus during early stage of infection. FnBPA was capable of promoting Staphylococcus aureus to invade cells and was viewed as a potential immune target. Based on the FnBPA-A gene two recombinant expression vectors with or without Kozak sequence were constructed. After identified and confirmed by restriction enzyme digestion and sequencing they were used to immunize C57BL/6 mice. Then induced antibody titer, T lymphocyte proliferative response and experiment mice challenge test were measured. Our result indicates that humoral immune responses and challenge experiment induced by recombinant DNA with Kozak sequence were better than those without Kozak sequence （P〈0.05）. For T lymphocyte proliferative response the induced effect of recombinant DNA sequence, but there was no significant difference （P〉0.05）. We in immune response induced by FnBPA-A recombinant DNA. with Kozak sequence was higher than that without Kozak conclude that Kozak sequence could play an important role

关 键 词：金黄色葡萄球菌 黏附因子FnBPA KOZAK序列 重组DNA疫苗 免疫应答 

分 类 号：S852.611[农业科学—基础兽医学]