双液相体系强化氧传递促进微生物油脂生产  

Improved production of microbial lipids in the two-liquid phase fermentation system

作　　者：颜日明[1] 艾佐佐[1,2] 汪涯[3] 张志斌[1] 曾庆桂[1] 朱笃[1,3] 

机构地区：[1]江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌330022 [2]江西教育学院理学院,江西南昌330032 [3]江西科技师范大学,江西南昌330013

出　　处：《生物工程学报》2013年第4期536-539,共4页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863计划)(No.2012AA021205);江西省高等学校科技落地计划;江西省主要学科学术与技术带头人培养计划(No.060002)资助~~

摘　　要：文中通过添加氧载体正十二烷进行双液相发酵来提高发酵性丝孢酵母利用木薯淀粉水解液生产微生物油脂的产量。结果表明,在摇瓶发酵液中添加氧载体,能明显缓解发酵过程中的氧限制程度。在2 L发酵罐中添加1%正十二烷进行双液相高密度发酵,其发酵生物量和油脂产量分别达到101.2 g/L和50.28 g/L。气相色谱分析表明,添加了氧载体发酵的微生物油脂中含有更高的饱和脂肪酸含量。In the present study, we developed a two-liquid phase fermentation system by adding 1% n-dodecane as oxygen-vector to enhance the microbial lipids productivity of Trichosporon fermentans using cassava starch hydrolysate. Results suggest that the oxygen-vector could alleviate the oxygen shortage in flask fermentation. The cell mass and lipids concentration were 101.2 g/L and 50.28 respectively in 2 L fermenter with the presence of 1% n-dodecane. Additionally,gas chromatography analysis also reveals that the microbial lipids produced by T. fermentans contained a higher percentage of saturated fatty acid in the oxygen-vector case.

关 键 词：正十二烷 氧载体 双液相发酵 微生物油脂 发酵性丝孢酵母 

分 类 号：Q939.97[生物学—微生物学]