温敏性壳聚糖止血膜止血作用的实验研究  

EXPERIMENTAL STUDY ON HEMOSTASIS OF THERMOSENSITIVE CHITOSAN HEMOSTATIC FILM

作　　者：尹刚[1] 魏长征 郭兴锋[1] 陈汇浩[1] 侯春林[1] 

机构地区：[1]上海长征医院骨科上海,200003 [2]上海其胜生物制剂有限公司

出　　处：《中国修复重建外科杂志》2013年第5期624-627,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的探讨温敏性壳聚糖止血膜的止血作用。方法取成年SD大鼠50只,雌雄不限,体重190～210 g,根据使用止血材料不同随机分为5组(n=10)。取各组大鼠制备肝创面出血模型后,分别采用相同大小(2.0 cm×1.0 cm×0.5 cm)的温敏性壳聚糖止血膜(A组)、壳聚糖止血膜(B组)、纤维素止血棉(C组)、明胶海绵(D组)覆盖切口,E组不作处理作为空白对照。大体观察各组出血情况,记录出血时间及出血量,并于4周后取各组肝脏愈合创面组织进行HE染色观察。结果大体观察A、B、C组止血效果较好、创面止血较快,D组止血效果较差、出血时间长,E组创面不能止血。A、B、C、D组止血时间和出血量均显著少于E组,A、B、C组均显著少于D组,差异有统计学意义(P<0.05);A、B、C组间比较差异均无统计学意义(P>0.05)。组织学观察示,与其余各组比较,A组肝细胞水肿较轻,轻度气球样变。结论温敏性壳聚糖止血膜对大鼠肝出血创面具有较好的止血作用。Objective To investigate the hemostasis of thermosensitive chitosan hemostatic film. Methods Fifty adult Sprague Dawley rats, male or female and weighing 190-210 g, were made the models of liver injury. The models were randomly divided into 5 groups （n=10） depending on different hemostatic materials. The incision of the liver was covered with the hemostatic materials of 2.0 cm × 1.0 cm ×0.5 cm in size： thermosensitive chitosan hemostatic film （group A）, chitosan hemostatic film （group B）, cellulose hemostatic cotton （group C）, gelatin sponge （group D）, and no treatment （group E）, respectively. The bleeding time and bleeding amount were recorded. After 4 weeks, the incisions of the liver were observed with HE staining. Results Gross observation showed better hemostatic effect and faster hemostatic time in groups A, B, and C; group D bad weaker hemostatic effect and slower hemostatic time; group E had no hemostatic effect. The bleeding time and bleeding amount of groups A, B, C, and D were significantly lower than those of group E （P 〈 0.05）. The bleeding time and bleeding amount of groups A, B, and C were significantly lower than those of group D （P 〈 0.05）, but no significant difference was found among groups A, B, and C （P 〉 0.05）. The liver cells of than other 4 groups through histological observation. Conclusion hemostasis effect on the 1 iver incision of rats. group A had milder edema and ballooning degeneration The thermosensitive chitosan hemostatic film has good

关 键 词：温敏性壳聚糖 生物止血材料 大鼠 

分 类 号：R318.08[医药卫生—生物医学工程]