可视化微囊化细胞共培养分离体系的建立  

Establishment of Visible Microencapsulated Cell Co-culture Separation System

作　　者：吴昊[1,2] 马颖[1,2] 单爽[1] 谭明乾[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院大学,北京100049

出　　处：《中国细胞生物学学报》2013年第5期697-702,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:91227126);中国科学院大连化学物理所"百人计划"支持的课题~~

摘　　要：细胞微囊化共培养在细胞诱导分化、细胞增殖调节和提高细胞代谢物产量方面是一种很有价值的技术。两种微囊化细胞的可视识别和分离在共培养过程中有重要作用。作为磁性介质,顺磁四氧化三铁纳米粒(Fe3O4nanoparticles,Fe3O4NPs)可用于实现对微囊的磁场驱动分离。该研究通过微胶囊膜内装载亲水四氧化三铁纳米粒(water soluble Fe3O4nanoparticles,W-Fe3O4NPs),同时使用荧光壳聚糖成膜,制备荧光可视顺磁性海藻酸–壳聚糖(alginate-chitosan,AC)微胶囊,用于中国仓鼠卵巢上皮细胞系(Chinese hamster ovary cell,CHO)细胞培养。结果表明,共培养细胞可以经荧光信号加以识别并利用磁场驱动加以分离。所构建的荧光可视顺磁性CHO细胞微胶囊在细胞微囊化共培养方面展现出较好的应用潜力。Cell microencapsulation co-culture has been proved to be a valuable technology in the fields of cell induced differentiation, cell proliferation regulation and improving the production of metabolites. Visible identification and separation of two kinds of microencapsulated cells play an important role in cell co-culture. Fe304 nanoparticles （Fe304 NPs） as a kind of paramagnetic material can be used for microencapsulated cell separation. In this study, microencapsulated cell co-culture system, fluorescent visible paramagnetic microcapsules were developed by doping water soluble Fe304 NPs into alginic acid-fluorescent chitosan microcapsules （AC microcapsules） membrane for co-culture of Chinese hamster ovary cell （CHO）. The results showed that two kinds of cell culture microcapsules could be identified by fluorescence signal and separated by magnetic driving in the process of cell co-culture. The fluorescent visible paramagnetic microcapsules system shows a great potential for cell co-culture.

关 键 词：细胞微囊化 细胞共培养 磁性驱动分离 荧光辨识 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]