斑马鱼肠黏膜屏障的结构与组成  

Structure and Composition of Intestinal Mucosal Barrier in Zebrafish （Brachydanio redo）

作　　者：章琳俐[1] 姚一琳[1] 初晓红[1] 张茜[1] 杨平[1] 卞勋光[1] 刘仪[1] 胡建华 高诚 林金杏 陈秋生[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]上海实验动物研究中心,上海201203

出　　处：《实验动物与比较医学》2013年第2期112-116,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科委资助项目（No.10140901100）

摘　　要：目的观察和分析斑马鱼肠黏膜屏障的结构与组成。方法应用透射电镜、组织化学以及免疫组织化学技术，系统观测和分析斑马鱼肠粘膜屏障的组织结构和细胞组成。结果斑马鱼肠道吸收细胞之间具有发达的连接复合体，自上而下依次可见紧密连接、中间连接和桥粒，它们构成封闲的机械屏障；肠黏膜上皮的吸收细胞之间分布着丰富的杯状细胞，尤以后肠的数量最多，PAS反应呈强阳性，其分泌物在肠腔面形成一层粘液层，为肠黏膜化学屏障的主要细胞；肠黏膜上皮内淋巴细胞和肥大细胞，为斑马鱼肠黏膜的主要免疫细胞。黏膜上皮中内分泌细胞既有开放型也有封闭型，纹状缘发达呈线状（或膜状）衬于肠上皮腔面。结论斑马鱼肠黏膜屏障系统由较完整的机械屏障、化学屏障和免疫屏障的结构组成，可作为肠黏膜屏障研究的动物模型。Objective To observe and analyze the structure and composition of intestinal mucosal barrier in zebrafish （Brachydanio rerio）. Methods The morphology was observed by transmission electron microscopy, histochemistry and immunohistochemistry were undertaken on the organizational structure and cell composition of intestinal mucosal barrier in the zebrafish. Results The zebrafish intestinal absorptive cells had developed junctional complex, top-down followed by visible tight junctions, intermediate connections, and desmosomes. They form a closed mechanical barrier. The intestinal mucosa absorption cell distributed between rich goblet cells, especially in the largest number of the intestine. PAS reaction was significant positive. The goblet cells were the chemical major cells for the intestinal mucosa barrier, whose secretions in the intestinal cavity surface to form a layer of mucus layer. Their intestinal intraepithelial lymphocytes and mast cells were the intestinal mucosa of primary immune cells. The mucosal epithelium of endocrine cells in both open and closed, striated border developed in a linear （or membrane） in the intestinal epithelium lining the cavity surface. Conclusions The intestinal mucosa barrier system of zebrafish consisted of relative complete mechanical barrier, chemical barrier and immune barrier structure. So zebrafish could be used as animal models for the study of intestinal mucosal barrier.

关 键 词：斑马鱼 肠道 黏膜屏障 结构 

分 类 号：Q95-33[生物学—动物学]