小型猪腹壁拉链装置的细胞毒性试验及排异反应观测  

The Cytotoxicity Test and Rejection Observation of Abdominal Zipper for Miniature Pigs

作　　者：余琛琳[1] 汤球[1] 刘志学[1] 马雷[1] 徐晨[1] 蔡丽萍[1] 崔淑芳[1] 

机构地区：[1]第二军医大学训练部实验动物中心,上海200433

出　　处：《实验动物与比较医学》2013年第2期130-134,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科技发展基金项目（08140901400,09140903100）

摘　　要：目的通过体外细胞毒性试验和在体排异反应试验，评价小型猪腹壁拉链装置的安全性。方法通过CCK-8法细胞毒性试验，评价小型猪腹壁拉链装置材料的细胞毒性；通过分别测定空白对照组（C组）、外科手术组（S组）和小型猪腹壁拉链模型组（M组）外科手术前（0d）及术后ld、3d、7d、14d和21d血清IL-1、IL-6、IL-10和TNF-a等免疫细胞因子的浓度变化，评价了小型猪腹壁拉链装置的安全性。结果细胞毒性为0-I级；IL-1、IL-6、IL．10和TNF-a值，M组和S组组问无显著性差异；M组和S组分别与C组相比较，术后ld和3d均显著升高、有极显著差异（P〈0．01），术后7d、14d、21d组间无显著性差异。结论小型猪腹壁拉链装置体外细胞毒性合格，符合生物材料的国家标准；在体排异反应试验表明生物拉链本身没有引起严重的排异反应或炎症反应，安全性良好，为小型猪腹壁拉链动物模型的建立和推广应用提供了良好的实验依据。Objective To assess the safety of abdominal zippers of miniature pig by cytotoxicity test and rejection observation. Methods The safety of abdominal zippers of miniature pig were evaluated by CCK-8 cytotoxicity test. In the test of rejection observation, nine miniature pigs were randomly divided into three groups： control group（C group）, surgical procedure group（S group） and the animal model of miniature pig with abdominal zipper group（M group）, and IL-1, IL-6, IL-10 and TNF-a in blood serum were detected before surgery （0 d）, and during the day of ld, 3 d, 7 d, 14 d and 21 d after surgery. Results The cytotoxicity grade was 0 or I level, and the level of IL-1, IL-6, IL-10 and TNF-a in S group and M group increased significantly at ld and 3 d after surgery, but there was no difference between S group, M group and C group at 7 d, 14 d and 21 d after surgery. Conclusions The abdominal zipper for miniature pigs, which satisfies the national safety standard of biological materials, has qualified cytotoxicity and excellent biosafety.

关 键 词：小型猪 生物拉链 细胞毒性试验 排异反应 

分 类 号：Q95-33[生物学—动物学]