肉苁蓉多糖对THP-1细胞吞噬作用的影响及其机理研究  

The Effects and Mechanism of Cistanche deserticola Polysaccharide on the THP-1 Cell’s Phagocytosis

作　　者：张涛[1] 许文胜[2] 贾彦斌[3] 白国荣[4] 高静[3] 崔丽霞[3] 郎力河[5] 张玉娟[5] 

机构地区：[1]内蒙古包头医学院免疫教研室,中国内蒙古包头014010 [2]内蒙古包头医学院第一附属医院骨科,中国内蒙古包头014010 [3]内蒙古包头医学院病原生物教研室,中国内蒙古包头014010 [4]内蒙古包头医学院第一附属医院蒙医骨伤科,中国内蒙古包头014010 [5]内蒙古包头医学院基础学院,中国内蒙古包头014010

出　　处：《生命科学研究》2013年第2期148-150,155,共4页Life Science Research

基　　金：内蒙古自治区教育厅资助项目(NJ10193);内蒙古卫生厅2010年医疗卫生科研计划项目(2010059);内蒙古自治区自然科学基金资助项目(2010MS1127)

摘　　要：将单核细胞株THP-1按照随机数字表随机分为观察组和对照组两组,正常的观察组给予肉苁蓉多糖培养液,根据肉苁蓉多糖浓度不同又分为10、50、100μg/L组,对照组不添加肉苁蓉多糖培养液,实验组中的不同浓度组、对照组,每组各24例.比较各组THP-1细胞吞噬率、吞噬指数以及细胞因子分泌水平,以此研究肉苁蓉多糖对THP-1细胞吞噬作用的影响并探讨其发生机理.结果显示,观察组中10、50、100μg/L组单核巨噬细胞吞噬率分别为(17.42±3.19)%、(23.32±3.82)%、(29.41±4.11)%,均高于对照组的(9.34±1.26)%,比较差异有统计学意义(F=162.63,P<0.01);10、50、100μg/L组单核巨噬细胞吞噬指数分别为(0.45±0.13)、(0.67±0.19)、(0.82±0.21),均高于对照组的(0.22±0.09),比较差异有统计学意义(F=62.60,P<0.01);10、50、100μg/L组IFN-γ分别为(4.21±1.11)、(7.38±1.86)、(9.51±2.10)mg/L,均高于对照组(0.79±0.06)mg/L,比较差异有统计学意义(F=152.74,P<0.01);10、50、100μg/L组IL-1分别为(24.11±4.15)、(74.60±11.21)、(103.61±19.65)mg/L,均高于对照组(10.21±1.39)mg/L,比较差异有统计学意义(F=343.15,P<0.01),各指标进一步采用Newman-Keals法进行两两比较均有统计学意义(P<0.01).由此认为,肉苁蓉多糖可能通过增强THP-1细胞吞噬能力和促细胞因子释放发挥免疫调节功能.The monocytic cell line THP-1 were randomly divided into observation group and control group. According to the different Cistanche desertico/a polysaccharide （CDPS） concentration, the observation groups were divided into 10, 50, 100 p,g/L groups; Control group without the addition of CDPS medium. 24 cases in the observation group and 24 cases in the control group. Comparison of THP-1 cell phagocytosis rate, phagocytic index and cytokine secretion levels to explore its mechanism and effects of Cistanche phagocytosis polysaccharides on THP-1 cells. The results showed： the phagocytic rate of 10, 50, 100 μg/L groups of mononuclear macrophage phagocytic rate were （17.42±3.19）%, （23.32±3.82）%, （29.41±4.11）%, higher than that of the control group （9.34±1.26）% with a statistically significant difference （F=162.63, P〈0.01）; The phagoeytosis indexes of 10, 50, 100 μg/L groups of mononuclear macrophage were（0.45±0.13）, （0.67±0.19）, （0.82± 0.21）, also higher than that of the control group （0.22±0.09） with a statistically significant difference （F=62.60, P〈 0.01）; IFN-T of 10, 50, 100 μg/L groups were（4.21_±1.11）, （7.38±1.86）, （9.51±2.10） mg/L, being higher than those in the control group （0.79_±0.06） mg/L with a statistically significant difference（F=152.74, P〈0.01）; Moreover, the value of IL-1 of 10, 50, 100 Ixg/L groups were （24.11±4.15）, （74.60±11.21）, （103.61±19.65） mg/k which were also higher than those in the control group （10.21±1.39） mg/L with a statistically significant difference（F=343.15, P〈0.01）. The difference of each index calculated further by using Newman-Keals method were again sta-tistieally significant difference（P〈0.01）. Therefore, it is concluded that Cistanche polysaeeharide may enhance the phagocytic ability of THP-1 eells and promote the release of cytokines in immune regulation function.

关 键 词：肉苁蓉多糖 THP-1细胞 吞噬作用 机理 

分 类 号：R551.3[医药卫生—血液循环系统疾病]